Recombinant Full Length Human L1CAM Protein, C-Flag-tagged

• Cat.No.: L1CAM-553HFL
• Product Overview: Recombinant Full Length Human L1CAM Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 137.8 kDa
• AA Sequence: MVVALRYVWPLLLCSPCLLIQIPEE YEGHHVMEPPVITEQSPRRLVVFPT DDISLKCEASGKPEVQFRWT RDGVHFKPKEELGVTVYQSPHSGSF TITGNNSNFAQRFQGIYRCFASNKL GTAMSHEIRLMAEGAPKWPK ETVKPVEVEEGESVVLPCNPPPSAE PLRIYWMNSKILHIKQDERVTMGQN GNLYFANVLTSDNHSDYICH AHFPGTRTIIQKEPIDLRVKATNSM IDRKPRLLFPTNSSSHLVALQGQPL VLECIAEGFPTPTIKWLRPS GPMPADRVTYQNHNKTLQLLKVGEE DDGEYRCLAENSLGSARHAYYVTVE AAPYWLHKPQSHLYGPGETA RLDCQVQGRPQPEVTWRINGIPVEE LAKDQKYRIQRGALILSNVQPSDTM VTQCEARNRHGLLLANAYIY VVQLPAKILTADNQTYMAVQGSTAY LLCKAFGAPVPSVQWLDEDGTTVLQ DERFFPYANGTLGIRDLQAN DTGRYFCLAANDQNNVTIMANLKVK DATQITQGPRSTIEKKGSRVTFTCQ ASFDPSLQPSITWRGDGRDL QELGDSDKYFIEDGRLVIHSLDYSD QGNYSCVASTELDVVESRAQLLVVG SPGPVPRLVLSDLHLLTQSQ VRVSWSPAEDHNAPIEKYDIEFEDK EMAPEKWYSLGKVPGNQTSTTLKLS PYVHYTFRVTAINKYGPGEP SPVSETVVTPEAAPEKNPVDVKGEG NETTNMVITWKPLRWMDWNAPQVQY RVQWRPQGTRGPWQEQIVSD PFLVVSNTSTFVPYEIKVQAVNSQG KGPEPQVTIGYSGEDYPQAIPELEG IEILNSSAVLVKWRPVDLAQ VKGHLRGYNVTYWREGSQRKHSKRH IHKDHVVVPANTTSVILSGLRPYSS YHLEVQAFNGRGSGPASEFT FSTPEGVPGHPEALHLECQSNTSLL LRWQPPLSHNGVLTGYVLSYHPLDE GGKGQLSFNLRDPELRTHNL TDLSPHLRYRFQLQATTKEGPGEAI VREGGTMALSGISDFGNISATAGEN YSVVSWVPKEGQCNFRFHIL FKALGEEKGGASLSPQYVSYNQSSY TQWDLQPDTDYEIHLFKERMFRHQM AVKTNGTGRVRLPPAGFATE GWFIGFVSAIILLLLVLLILCFIKR SKGGKYSVKDKEDTQVDSEARPMKD ETFGEYRSLESDNEEKAFGS SQPSLNGDIKPLGSDDSLADYGGSV DVQFNEDGSFIGQYSGKKEKEAAGG NDSSGATSPINPAVALETRTRPLEQ KLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Axon guidance, Cell adhesion molecules (CAMs)

Gene Information

• Gene Name: L1CAM L1 cell adhesion molecule [ Homo sapiens (human) ]
• Official Symbol: L1CAM
• Synonyms: S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
• Gene ID: 3897
• mRNA Refseq: NM_000425.5
• Protein Refseq: NP_000416.1
• MIM: 308840
• UniProt ID: P32004

Reviews

04/11/2020

L1CAM protein is an enzyme involved in the digestion of carbohydrates, specifically starch.

07/07/2019

A reliable manufacturer ensures the production and purification of L1CAM protein, maintaining its quality and purity.

10/08/2017

Manufacturers should provide detailed information about the production process, quality control measures, and purity assessment methods employed.

Q&As

Are there any L1CAM-targeted therapies in clinical trials?

Some clinical trials are exploring L1CAM-targeted therapies in the context of cancer treatment.

Can L1CAM be used as a diagnostic marker for neurological disorders?

L1CAM mutations can be diagnosed through genetic testing, aiding in the diagnosis of L1 syndrome and related conditions.

Can L1CAM be a therapeutic target in cancer treatment?

L1CAM has been explored as a potential therapeutic target for inhibiting tumor metastasis in some cancer types.

Are there clinical applications for L1CAM in neurodegenerative diseases?

L1CAM is not typically associated with neurodegenerative diseases, but its role in neural development may have implications for regenerative medicine.

In what other medical conditions is L1CAM implicated?

L1CAM has been associated with certain developmental disorders and cognitive deficits in addition to cancer and neurological disorders.