Recombinant Full Length Human L3HYPDH Protein, C-Flag-tagged

• Cat.No.: L3HYPDH-1699HFL
• Product Overview: Recombinant Full Length Human L3HYPDH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a dehydratase that converts trans-3-hydroxy-L-proline to delta(1)-pyrroline-2-carboxylate. This enzyme may function to degrade dietary proteins that contain trans-3-hydroxy-L-proline as well as other proteins such as collagen IV. The encoded protein can be converted to an epimerase by changing a threonine to a cysteine at a catalytic site.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 38 kDa
• AA Sequence: MESALAVPWLPPHDPGTPVLSVVDM HTGGEPLRIVLAGCPEVSGPTLLAK RRYMRQHLDHVRRRLMFEPR GHRDMYGAVLVPSELPDAHLGVLFL HNEGYSSMCGHAVLALGRFALDFGL VPAPPAGTREARVNIHCPCG LVTAFVACEDGRSHGPVRFHSVPAF VLATDLMVDVPGHGKVMVDIAYGGA FYAFVTAEKLGLDICSAKTR DLVDAASAVTEAVKAQFKINHPDSE DLAFLYGTILTDGKDAYTKEPTTNI CVFADEQVDRSPTGSGVTAR IALQYHKGLLELNQMRAFKSSATGS VFTGKAVREAKCGDFKAVIVEVSGQ AHYTGTASFIIEDDDPLRDG
  FLLKTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: L3HYPDH trans-L-3-hydroxyproline dehydratase [ Homo sapiens (human) ]
• Official Symbol: L3HYPDH
• Synonyms: C14orf149
• Gene ID: 112849
• mRNA Refseq: NM_144581.2
• Protein Refseq: NP_653182.1
• MIM: 614811
• UniProt ID: Q96EM0