Recombinant Full Length Human LIPE Protein, C-Flag-tagged

• Cat.No.: LIPE-713HFL
• Product Overview: Recombinant Full Length Human LIPE Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 116.4 kDa
• AA Sequence: MEPGSKSVSRSDWQPEPHQRPITPL EPGPEKTPIAQPESKTLQGSNTQQK PASNQRPLTQQETPAQHDAE SQKEPRAQQKSASQEEFLAPQKPAP QQSPYIQRVLLTQQEAASQQGPGLG KESITQQEPALRQRHVAQPG PGPGEPPPAQQEAESTPAAQAKPGA KREPSAPTESTSQETPEQSDKQTTP VQGAKSKQGSLTELGFLTKL QELSIQRSALEWKALSEWVTDSESE SDVGSSSDTDSPATMGGMVAQGVKL GFKGKSGYKVMSGYSGTSPH EKTSARNHRHYQDTASRLIHNMDLR TMTQSLVTLAEDNIAFFSSQGPGET AQRLSGVFAGVREQALGLEP ALGRLLGVAHLFDLDPETPANGYRS LVHTARCCLAHLLHKSRYVASNRRS IFFRTSHNLAELEAYLAALT QLRALVYYAQRLLVTNRPGVLFFEG DEGLTADFLREYVTLHKGCFYGRCL GFQFTPAIRPFLQTIYIGLV SFGEHYKRNETGLSVAASSLFTSGR FAIDPELRGAEFERITQNLDVHFWK AFWNITEMEVLSSLANMASA TVRVSRLLSLPPEAFEMPLTADPTL TVTISPPLAHTGPGPVLVRLISCDL REGQDSEELSSLIKSNGQRS LELWPRPQQAPRSRSLIVHFHGGGF VAQTSRSHEPYLKSWAQELGAPIIS IDYSLAPEAPFPRALEECFF AYCWAIKHCALLGSTGERICLAGDS AGGNLCFTVALRAAAYGVRVPDGIM AAYPATMLQPAASPSRLLSL MDPLLPLSVLSKCVSAYAGAKTEDH SNSDQKALGMMGLVRRDTALLLRDF RLGASSWLNSFLELSGRKSQ KMSEPIAEPMRRSVSEAALAQPQGP LGTDSLKNLTLRDLSLRGNSETSSD TPEMSLSAETLSPSTPSDVN FLLPPEDAGEEAEAKNELSPMDRGL GVRAAFPEGFHPRRSSQGATQMPLY SSPIVKNPFMSPLLAPDSML KSLPPVHIVACALDPMLDDSVMLAR RLRNLGQPVTLRVVEDLPHGFLTLA ALCRETRQAAELCVERIRLV
  LTPPAGAGPSGETGAAGVDGGCGGR HTRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Insulin signaling pathway

Gene Information

• Gene Name: LIPE lipase E, hormone sensitive type [ Homo sapiens (human) ]
• Official Symbol: LIPE
• Synonyms: HSL; LHS; REH; AOMS4; FPLD6
• Gene ID: 3991
• mRNA Refseq: NM_005357.4
• Protein Refseq: NP_005348.2
• MIM: 151750
• UniProt ID: Q05469