Recombinant Full Length Human LMNB2 Protein, C-Flag-tagged

• Cat.No.: LMNB2-1361HFL
• Product Overview: Recombinant Full Length Human LMNB2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 67.5 kDa
• AA Sequence: MSPPSPGRRREQRRPRAAATMATPL PGRAGGPATPLSPTRLSRLQEKEEL RELNDRLAHYIDRVRALEL ENDRLLLKISEKEEVTTREVSGIKA LYESELADARRVLDETARERARLQI EIGKLRAELDEVNKSAKKR EGELTVAQGRVKDLESLFHRSEVEL AAALSDKRGLESDVAELRAQLAKAE DGHAVAKKQLEKETLMRVD LENRCQSLQEELDFRKSVFEEEVRE TRRRHERRLVEVDSSRQQEYDFKMA QALEELRSQHDEQVRLYKL ELEQTYQAKLDSAKLSSDQNDKAAS AAREELKEARMRLESLSYQLSGLQK QASAAEDRIRELEEAMAGE RDKFRKMLDAKEQEMTEMRDVMQQQ LAEYQELLDVKLALDMEINAYRKLL EGEEERLKLSPSPSSRVTV SRATSSSSGSLSATGRLGRSKRKRL EVEEPLGSGPSVLGTGTGGSGGFHL AQQASASGSVSIEEIDLEG KFVQLKNNSDKDQSLGNWRIKRQVL EGEEIAYKFTPKYILRAGQMVTVWA AGAGVAHSPPSTLVWKGQS SWGTGESFRTVLVNADGEEVAMRTV KKSSVMRENENGEEEEEEAEFGEED LFHQQGDPRTTSRGCYVMTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: LMNB2 lamin B2 [ Homo sapiens (human) ]
• Official Symbol: LMNB2
• Synonyms: EPM9; LMN2; LAMB2; MCPH27
• Gene ID: 84823
• mRNA Refseq: NM_032737.4
• Protein Refseq: NP_116126.3
• MIM: 150341
• UniProt ID: Q03252