Recombinant Full Length Human MAPK7 Protein, C-Flag-tagged

• Cat.No.: MAPK7-2125HFL
• Product Overview: Recombinant Full Length Human MAPK7 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors. In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 88.2 kDa
• AA Sequence: MAEPLKEEDGEDGSAEPPGPVKAEP AHTAASVAAKNLALLKARSFDVTFD VGDEYEIIETIGNGAYGVVS SARRRLTGQQVAIKKIPNAFDVVTN AKRTLRELKILKHFKHDNIIAIKDI LRPTVPYGEFKSVYVVLDLM ESDLHQIIHSSQPLTLEHVRYFLYQ LLRGLKYMHSAQVIHRDLKPSNLLV NENCELKIGDFGMARGLCTS PAEHQYFMTEYVATRWYRAPELMLS LHEYTQAIDLWSVGCIFGEMLARRQ LFPGKNYVHQLQLIMMVLGT PSPAVIQAVGAERVRAYIQSLPPRQ PVPWETVYPGADRQALSLLGRMLRF EPSARISAAAALRHPFLAKY HDPDDEPDCAPPFDFAFDREALTRE RIKEAIVAEIEDFHARREGIRQQIR FQPSLQPVASEPGCPDVEMP SPWAPSGDCAMESPPPAPPPCPGPA PDTIDLTLQPPPPVSEPAPPKKDGA ISDNTKAALKAALLKSLRSR LRDGPSAPLEAPEPRKPVTAQERQR EREEKRRRRQERAKEREKRRQERER KERGAGASGGPSTDPLAGLV LSDNDRSLLERWTRMARPAAPALTS VPAPAPAPTPTPTPVQPTSPPPGPV AQPTGPQPQSAGSTSGPVPQ PACPPPGPAPHPTGPPGPIPVPAPP QIATSTSLLAAQSLVPPPGLPGSST PGVLPYFPPGLPPPDAGGAP QSSMSESPDVNLVTQQLSKSQVEDP LPPVFSGTPKGSGAGYGVGFDLEEF LNQSFDMGVADGPQDGQADS ASLSASLLADWLEGHGMNPADIESL QREIQMDSPMLLADLPDLQDP TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, GnRH signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway

Gene Information

• Gene Name: MAPK7 mitogen-activated protein kinase 7 [ Homo sapiens (human) ]
• Official Symbol: MAPK7
• Synonyms: BMK1; ERK4; ERK5; PRKM7
• Gene ID: 5598
• mRNA Refseq: NM_002749.4
• Protein Refseq: NP_002740.2
• MIM: 602521
• UniProt ID: Q13164