Recombinant Full Length Human MICA Protein, C-Flag-tagged

• Cat.No.: MICA-746HFL
• Product Overview: Recombinant Full Length Human MICA Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 38.3 kDa
• AA Sequence: MGLGPVFLLLAGIFPFAPPGAAAEP HSLRYNLTVLSWDGSVQSGFLAEVH LDGQPFLRYDRQKCRAKPQG QWAEDVLGNKTWDRETRDLTGNGKD LRMTLAHIKDQKEGLHSLQEIRVCE IHEDNSTRSSQHFYYDGELF LSQNLETEEWTVPQSSRAQTLAMNV RNFLKEDAMKTKTHYHAMHADCLQE LRRYLESGVVLRRTVPPMVN VTRSEASEGNITVTCRASSFYPRNI ILTWRQDGVSLSHDTQQWGDVLPDG NGTYQTWVATRICRGEEQRF
  TCYMEHSGNHSTHPVPSGKVLVLQS HWQTFHVSAVAAGCCYFCYYYFLCP LLTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: MICA MHC class I polypeptide-related sequence A [ Homo sapiens (human) ]
• Official Symbol: MICA
• Synonyms: MIC-A; PERB11.1
• Gene ID: 100507436
• mRNA Refseq: NM_001177519.3
• Protein Refseq: NP_001170990.1
• MIM: 600169
• UniProt ID: Q96QC4