Recombinant Full Length Human MMP2 Protein, C-Flag-tagged

• Cat.No.: MMP2-1611HFL
• Product Overview: Recombinant Full Length Human MMP2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70.9 kDa
• AA Sequence: MEALMARGALTGPLRALCLLGCLLS HAAAAPSPIIKFPGDVAPKTDKELA VQYLNTFYGCPKESCNLFVL KDTLKKMQKFFGLPQTGDLDQNTIE TMRKPRCGNPDVANYNFFPRKPKWD KNQITYRIIGYTPDLDPETV DDAFARAFQVWSDVTPLRFSRIHDG EADIMINFGRWEHGDGYPFDGKDGL LAHAFAPGTGVGGDSHFDDD ELWTLGEGQVVRVKYGNADGEYCKF PFLFNGKEYNSCTDTGRSDGFLWCS TTYNFEKDGKYGFCPHEALF TMGGNAEGQPCKFPFRFQGTSYDSC TTEGRTDGYRWCGTTEDYDRDKKYG FCPETAMSTVGGNSEGAPCV FPFTFLGNKYESCTSAGRSDGKMWC ATTANYDDDRKWGFCPDQGYSLFLV AAHEFGHAMGLEHSQDPGAL MAPIYTYTKNFRLSQDDIKGIQELY GASPDIDLGTGPTPTLGPVTPEICK QDIVFDGIAQIRGEIFFFKD RFIWRTVTPRDKPMGPLLVATFWPE LPEKIDAVYEAPQEEKAVFFAGNEY WIYSASTLERGYPKPLTSLG LPPDVQRVDAAFNWSKNKKTYIFAG DKFWRYNEVKKKMDPGFPKLIADAW NAIPDNLDAVVDLQGGGHSY
  FFKGAYYLKLENQSLKSVKFGSIKS DWLGCTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Bladder cancer, GnRH signaling pathway, Leukocyte transendothelial migration, Pathways in cancer

Gene Information

• Gene Name: MMP2 matrix metallopeptidase 2 [ Homo sapiens (human) ]
• Official Symbol: MMP2
• Synonyms: CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
• Gene ID: 4313
• mRNA Refseq: NM_004530.6
• Protein Refseq: NP_004521.1
• MIM: 120360
• UniProt ID: P08253