Recombinant Full Length Human PAPSS1 Protein, His tagged

• Cat.No.: PAPSS1-12HFL
• Product Overview: Recombinant Full Length Human PAPSS1 Protein with His tag was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-624 aa
• Description: PAPSS1 is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity. In the N-terminal section, it belongs to the APS kinase family; while the C-terminal section belongs to the sulfate adenylyltransferase family. PAPSS1 can be inhibited by chlorate, and is expressed in many tissues, such as high endothelial venules (HEV) cells and in cartilage. PAPSS1 mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate. In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. PAPSS1 also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
• Form: Liquid
• Molecular Mass: 71.9 kD
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Application: This recombinant protein can be used for biological assays. For research use only.
• Storage: Store at -20 centigrade, stable for 6 months after receipt. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Storage Buffer: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl and 20% glycerol. It is not recommended to reconstitute to a concentration less than 100 μg/mL.

Gene Information

• Gene Name: PAPSS1 3-phosphoadenosine 5-phosphosulfate synthase 1 [ Homo sapiens (human) ]
• Official Symbol: PAPSS1
• Synonyms: PAPSS1; 3-phosphoadenosine 5-phosphosulfate synthase 1; bifunctional 3-phosphoadenosine 5-phosphosulfate synthase 1; ATPSK1; PAPSS; SK 1; PAPSS 1; PAPS synthase 1; sulfurylase kinase 1; 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1; SK1
• Gene ID: 9061
• mRNA Refseq: NM_005443
• Protein Refseq: NP_005434
• MIM: 603262
• UniProt ID: O43252