Recombinant Full Length Human PDE1B Protein, C-Flag-tagged

• Cat.No.: PDE1B-704HFL
• Product Overview: Recombinant Full Length Human PDE1B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 61.2 kDa
• AA Sequence: MELSPRSPPEMLEESDCPSPLELKS APSKKMWIKLRSLLRYMVKQLENGE INIEELKKNLEYTASLLEAV YIDETRQILDTEDELQELRSDAVPS EVRDWLASTFTQQARAKGRRAEEKP KFRSIVHAVQAGIFVERMFR RTYTSVGPTYSTAVLNCLKNLDLWC FDVFSLNQAADDHALRTIVFELLTR HNLISRFKIPTVFLMSFLDA LETGYGKYKNPYHNQIHAADVTQTV HCFLLRTGMVHCLSEIELLAIIFAA AIHDYEHTGTTNSFHIQTKS ECAIVYNDRSVLENHHISSVFRLMQ DDEMNIFINLTKDEFVELRALVIEM VLATDMSCHFQQVKTMKTAL QQLERIDKPKALSLLLHAADISHPT KQWLVHSRWTKALMEEFFRQGDKEA ELGLPFSPLCDRTSTLVAQS QIGFIDFIVEPTFSVLTDVAEKSVQ PLADEDSKSKNQPSFQWRQPSLDVE VGDPNPDVVSFRSTWVKRIQ
  ENKQKWKERAASGITNQMSIDELSP CEEEAPPSPAEDEHNQNGNLDTRTR PLEQKLISEEDLAANDILDYKDDDD KV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism

Gene Information

• Gene Name: PDE1B phosphodiesterase 1B [ Homo sapiens (human) ]
• Official Symbol: PDE1B
• Synonyms: PDE1B1; PDES1B; HEL-S-79p
• Gene ID: 5153
• mRNA Refseq: NM_000924.4
• Protein Refseq: NP_000915.1
• MIM: 171891
• UniProt ID: Q01064