Recombinant Full Length Human PIAS4 Protein, C-Flag-tagged

• Cat.No.: PIAS4-1442HFL
• Product Overview: Recombinant Full Length Human PIAS4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Enables SUMO ligase activity and ubiquitin protein ligase binding activity. Involved in negative regulation of transcription, DNA-templated; positive regulation of protein sumoylation; and protein sumoylation. Located in cytoplasm and nucleus.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 56.3 kDa
• AA Sequence: MAAELVEAKNMVMSFRVSDLQMLLG FVGRSKSGLKHELVTRALQLVQFDC SPELFKKIKELYETRYAKKN SEPAPQPHRPLDPLTMHSTYDRAGA VPRTPLAGPNIDYPVLYGKYLNGLG RLPAKTLKPEVRLVKLPFFN MLDELLKPTELVPQNNEKLQESPCI FALTPRQVELIRNSRELQPGVKAVQ VVLRICYSDTSCPQEDQYPP NIAVKVNHSYCSVPGYYPSNKPGVE PKRPCRPINLTHLMYLSSATNRITV TWGNYGKSYSVALYLVRQLT SSELLQRLKTIGVKHPELCKALVKE KLRLDPDSEIATTGVRVSLICPLVK MRLSVPCRAETCAHLQCFDA VFYLQMNEKKPTWMCPVCDKPAPYD QLIIDGLLSKILSECEDADEIEYLV DGSWCPIRAEKERSCSPQGA ILVLGPSDANGLLPAPSVNGSGALG STGGGGPVGSMENGKPGADVVDLTL DSSSSSEDEEEEEEEEEDED
  EEGPRPKRRCPFQKGLVPACTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: PIAS4 protein inhibitor of activated STAT 4 [ Homo sapiens (human) ]
• Official Symbol: PIAS4
• Synonyms: PIASY; Piasg; ZMIZ6; PIAS-gamma
• Gene ID: 51588
• mRNA Refseq: NM_015897.4
• Protein Refseq: NP_056981.2
• MIM: 605989
• UniProt ID: Q8N2W9