| Description : |
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
56.5 kDa |
| AA Sequence : |
MAAELVEAKNMVMSFRVSDLQMLLG FVGRSKSGLKHELVTRALQLVQFDC SPELFKKIKELYETRYAKKNSEPAP QPHRPLDPLTMHSTYDRAGAVPRTP LAGPNIDYPVLYGKYLNGLGRLPAK TLKPEVRLVKLPFFNMLDELLKPTE LVPQNNEKLQESPCIFALTPRQVEL IRNSRELQPGVKAVQVVLRICYSDT SCPQEDQYPPNIAVKVNHSYCSVPG YYPSNKPGVEPKRPCRPINLTHLMY LSSATNRITVTWGNYGKSYSVALYL VRQLTSSELLQRLKTIGVKHPELCK ALVKEKLRLDPDSEIATTGVRVSLI CPLVKMRLSVPCRAETCAHLQCFDA VFYLQMNEKKPTWMCPVCDKPAPYD QLIIDGLLSKILSECEDADEIEYLV DGSWCPIRAEKERSCSPQGAILVLG PSDANGLLPAPSVNGSGALGSTGGG GPVGSMENGKPGADVVDLTLDSSSS SEDEEEEEEEEEDEDEEGPRPKRRC PFQKGLVPACTRTRPLEQKLISEED LAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
