Recombinant Full Length Human PKMYT1 Protein, C-Flag-tagged

• Cat.No.: PKMYT1-918HFL
• Product Overview: Recombinant Full Length Human PKMYT1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.3 kDa
• AA Sequence: MLERPPALAMPMPTEGTPPPLSGTP IPVPAYFRHAEPGFSLKRPRGLSRS LPPPPPAKGSIPISRLFPPR TPGWHQLQPRRVSFRGEASETLQSP GYDPSRPESFFQQSFQRLSRLGHGS YGEVFKVRSKEDGRLYAVKR SMSPFRGPKDRARKLAEVGSHEKVG QHPCCVRLEQAWEEGGILYLQTELC GPSLQQHCEAWGASLPEAQV WGYLRDTLLALAHLHSQGLVHLDVK PANIFLGPRGRCKLGDFGLLVELGT AGAGEVQEGDPRYMAPELLQ GSYGTAADVFSLGLTILEVACNMEL PHGGEGWQQLRQGYLPPEFTAGLSS ELRSVLVMMLEPDPKLRATA EALLALPVLRQPRAWGVLWCMAAEA LSRGWALWQALLALLCWLWHGLAHP ASWLQPLGPPATPPGSPPCS LLLDSSLSSNWDDDSLGPSLSPEAV LARTVGSTSTPRSRCTPRDALDLSD INSEPPRGSFPSFEPRNLLS
  LFEDTLDPTTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation

Gene Information

• Gene Name: PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 [ Homo sapiens (human) ]
• Official Symbol: PKMYT1
• Synonyms: MYT1; PPP1R126
• Gene ID: 9088
• mRNA Refseq: NM_004203.5
• Protein Refseq: NP_004194.3
• MIM: 602474
• UniProt ID: Q99640