Recombinant Full Length Human PMEL Protein, C-Flag-tagged

• Cat.No.: PMEL-835HFL
• Product Overview: Recombinant Full Length Human PMEL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70.1 kDa
• AA Sequence: MDLVLKRCLLHLAVIGALLAVGATK VPRNQDWLGVSRQLRTKAWNRQLYP EWTEAQRLDCWRGGQVSLKV SNDGPTLIGANASFSIALNFPGSQK VLPDGQVIWVNNTIINGSQVWGGQP VYPQETDDACIFPDGGPCPS GSWSQKRSFVYVWKTWGQYWQVLGG PVSGLSIGTGRAMLGTHTMEVTVYH RRGSRSYVPLAHSSSAFTIT DQVPFSVSVSQLRALDGGNKHFLRN QPLTFALQLHDPSGYLAEADLSYTW DFGDSSGTLISRALVVTHTY LEPGPVTAQVVLQAAIPLTSCGSSP VPGTTDGHRPTAEAPNTTAGQVPTT EVVGTTPGQAPTAEPSGTTS VQVPTTEVISTAPVQMPTAESTGMT PEKVPVSEVMGTTLAEMSTPEATGM TPAEVSIVVLSGTTAAQVTT TEWVETTARELPIPEPEGPDASSIM STESITGSLGPLLDGTATLRLVKRQ VPLDCVLYRYGSFSVTLDIV QGIESAEILQAVPSGEGDAFELTVS CQGGLPKEACMEISSPGCQPPAQRL CQPVLPSPACQLVLHQILKG GSGTYCLNVSLADTNSLAVVSTQLI MPGQEAGLGQVPLIVGILLVLMAVV LASLIYRRRLMKQDFSVPQL
  PHSSSHWLRLPRIFCSCPIGENSPL LSGQQVTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Secreted Protein, Transmembrane

Gene Information

• Gene Name: PMEL premelanosome protein [ Homo sapiens (human) ]
• Official Symbol: PMEL
• Synonyms: P1; SI; SIL; ME20; P100; SILV; HMB45; ME20M; gp100; HMB-45; ME20-M; PMEL17; D12S53E
• Gene ID: 6490
• mRNA Refseq: NM_006928.5
• Protein Refseq: NP_008859.1
• MIM: 155550
• UniProt ID: P40967