| Description : |
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms. |
| Source : |
Mammalian cells |
| Species : |
Human |
| Tag : |
Flag |
| Form : |
25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
| Molecular Mass : |
47 kDa |
| AA Sequence : |
MTAIIKEIVSRNKRRYQEDGFDLDL TYIYPNIIAMGFPAERLEGVYRNNI DDVVRFLDSKHKNHYKIYNL CAERHYDTAKFNCRVAQYPFEDHNP PQLELIKPFCEDLDQWLSEDDNHVA AIHCKAGKGRTGVMICAYLL HRGKFLKAQEALDFYGEVRTRDKKG VTIPSQRRYVYYYSYLLKNHLDYRP VALLFHKMMFETIPMFSGGT CNPQFVVCQLKVKIYSSNSGPTRRE DKFMYFEFPQPLPVCGDIKVEFFHK QNKMLKKDKMFHFWVNTFFI PGPEETSEKVENGSLCDQEIDSICS IERADNDKEYLVLTLTKNDLDKANK DKANRYFSPNFKVKLYFTKT
VEEPSNPEASSSTSVTPDVSDNEPD HYRYSDTTDSDPENEPFDEDQHTQI TKVTRTRPLEQKLISEEDLAANDIL DYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining. |
| Stability : |
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
>50 ug/mL as determined by microplate BCA method. |
| Preparation : |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Protein Families : |
Druggable Genome, Phosphatase |
| Protein Pathways : |
Endometrial cancer, Focal adhesion, Glioma, Inositol phosphate metabolism, Melanoma, p53 signaling pathway, Pathways in cancer, Phosphatidylinositol signaling system, Prostate cancer, Small cell lung cancer, Tight junction |
