Recombinant Full Length Human RAD51 Protein, C-Flag-tagged

• Cat.No.: RAD51-279HFL
• Product Overview: Recombinant Full Length Human RAD51 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 36.8 kDa
• AA Sequence: MAMQMQLEANADTSVEEESFGPQPI SRLEQCGINANDVKKLEEAGFHTVE AVAYAPKKELINIKGISEAK ADKILAEAAKLVPMGFTTATEFHQR RSEIIQITTGSKELDKLLQGGIETG SITEMFGEFRTGKTQICHTL AVTCQLPIDRGGGEGKAMYIDTEGT FRPERLLAVAERYGLSGSDVLDNVA YARAFNTDHQTQLLYQASAM MVESRYALLIVDSATALYRTDYSGR GELSARQMHLARFLRMLLRLADEFG VAVVITNQVVAQVDGAAMFA
  ADPKKPIGGNIIAHASTTRLYLRKG RGETRICKIYDSPCLPEAEAMFAIN ADGVGDAKDTRTRPLEQKLISEEDL AANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Homologous recombination, Pancreatic cancer, Pathways in cancer

Gene Information

• Gene Name: RAD51 RAD51 recombinase [ Homo sapiens (human) ]
• Official Symbol: RAD51
• Synonyms: RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
• Gene ID: 5888
• mRNA Refseq: NM_002875.5
• Protein Refseq: NP_002866.2
• MIM: 179617
• UniProt ID: Q06609