Recombinant Full Length Human RASGRP1 Protein, C-Flag-tagged

• Cat.No.: RASGRP1-556HFL
• Product Overview: Recombinant Full Length Human RASGRP1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 90.2 kDa
• AA Sequence: MGTLGKAREAPRKPSHGCRAASKAR LEAKPANSPFPSHPSLAHITQFRMM VSLGHLAKGASLDDLIDSCI QSFDADGNLCRSNQLLQVMLTMHRI VISSAELLQKVITLYKDALAKNSPG LCLKICYFVRYWITEFWVMF KMDASLTDTMEEFQELVKAKGEELH CRLIDTTQINARDWSRKLTQRIKSN TSKKRKVSLLFDHLEPEELS EHLTYLEFKSFRRISFSDYQNYLVN SCVKENPTMERSIALCNGISQWVQL MVLSRPTPQLRAEVFIKFIQ VAQKLHQLQNFNTLMAVIGGLCHSS ISRLKETSSHVPHEINKVLGEMTEL LSSSRNYDNYRRAYGECTDF KIPILGVHLKDLISLYEAMPDYLED GKVNVHKLLALYNHISELVQLQEVA PPLEANKDLVHLLTLSLDLY YTEDEIYELSYAREPRNHRAPPLTP SKPPVVVDWASGVSPKPDPKTISKH VQRMVDSVFKNYDHDQDGYI SQEEFEKIAASFPFSFCVMDKDREG LISRDEITAYFMRASSIYSKLGLGF PHNFQETTYLKPTFCDNCAG FLWGVIKQGYRCKDCGMNCHKQCKD LVVFECKKRAKNPVAPTENNTSVGP VSNLCSLGAKDLLHAPEEGP FTFPNGEAVEHGEESKDRTIMLMGV SSQKISLRLKRAVAHKATQTESQPW IGSEGPSGPFVLSSPRKTAQ DTLYVLPSPTSPCPSPVLVRKRAFV KWENKDSLIKSKEELRHLRLPTYQE LEQEINTLKADNDALKIQLK
  YAQKKIESLQLEKSNHVLAQMEQGD CSTRTRPLEQKLISEEDLAANDILD YKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: MAPK signaling pathway, T cell receptor signaling pathway

Gene Information

• Gene Name: RASGRP1 RAS guanyl releasing protein 1 [ Homo sapiens (human) ]
• Official Symbol: RASGRP1
• Synonyms: IMD64; RASGRP; CALDAG-GEFI; CALDAG-GEFII
• Gene ID: 10125
• mRNA Refseq: NM_005739.4
• Protein Refseq: NP_005730.2
• MIM: 603962
• UniProt ID: O95267