Recombinant Full Length Human RCN1 Protein, C-Flag-tagged

• Cat.No.: RCN1-2142HFL
• Product Overview: Recombinant Full Length Human RCN1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 35.8 kDa
• AA Sequence: MARGGRGRRLGLALGLLLALVLAPR VLRAKPTVRKERVVRPDSELGERPP EDNQSFQYDHEAFLGKEDSK TFDQLTPDESKERLGKIVDRIDNDG DGFVTTEELKTWIKRVQKRYIFDNV AKVWKDYDRDKDDKISWEEY KQATYGYYLGNPAEFHDSSDHHTFK KMLPRDERRFKAADLNGDLTATREE FTAFLHPEEFEHMKEIVVLE TLEDIDKNGDGFVDQDEYIADMFSH EENGPEPDWVLSEREQFNEFRDLNK DGKLDKDEIRHWILPQDYDH AQAEARHLVYESDKNKDEKLTKEEI LENWNMFVGSQATNYGEDLTKNHDE L TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: RCN1 reticulocalbin 1 [ Homo sapiens (human) ]
• Official Symbol: RCN1
• Synonyms: RCN; RCAL; PIG20; HEL-S-84
• Gene ID: 5954
• mRNA Refseq: NM_002901.4
• Protein Refseq: NP_002892.1
• MIM: 602735
• UniProt ID: Q15293