Recombinant Full Length Human RPN1 Protein, C-Flag-tagged

• Cat.No.: RPN1-1186HFL
• Product Overview: Recombinant Full Length Human RPN1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 66.2 kDa
• AA Sequence: MEAPAAGLFLLLLLGTWAPAPGSAS SEAPPLINEDVKRTVDLSSHLAKVT AEVVLAHLGGGSTSRATSFL LALEPELEARLAHLGVQVKGEDEEE NNLEVRETKIKGKSGRFFTVKLPVA LDPGAKISVIVETVYTHVLH PYPTQITQSEKQFVVFEGNHYFYSP YPTKTQTMRVKLASRNVESYTKLGN PTRSEDLLDYGPFRDVPAYS QDTFKVHYENNSPFLTITSMTRVIE VSHWGNIAVEENVDLKHTGAVLKGP FSRYDYQRQPDSGISSIRSF KTILPAAAQDVYYRDEIGNVSTSHL LILDDSVEMEIRPRFPLFGGWKTHY IVGYNLPSYEYLYNLGDQYA LKMRFVDHVFDEQVIDSLTVKIILP EGAKNIEIDSPYEISRAPDELHYTY LDTFGRPVIVAYKKNLVEQH IQDIVVHYTFNKVLMLQEPLLVVAA FYILFFTVIIYVRLDFSITKDPAAE ARMKVACITEQVLTLVNKRI GLYRHFDETVNRYKQSRDISTLNSG KKSLETEHKALTSEIALLQSRLKTE GSDLCDRVSEMQKLDAQVKE
  LVLKSAVEAERLVAGKLKKDTYIEN EKLISGKRQELVTKIDHILDALTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis

Gene Information

• Gene Name: RPN1 ribophorin I [ Homo sapiens (human) ]
• Official Symbol: RPN1
• Synonyms: OST1; RBPH1
• Gene ID: 6184
• mRNA Refseq: NM_002950.4
• Protein Refseq: NP_002941.1
• MIM: 180470
• UniProt ID: P04843