Recombinant Full Length Human RUVBL2 Protein, C-Flag-tagged

• Cat.No.: RUVBL2-1181HFL
• Product Overview: Recombinant Full Length Human RUVBL2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 51 kDa
• AA Sequence: MATVTATTKVPEIRDVTRIERIGAH SHIRGLGLDDALEPRQASQGMVGQL AARRAAGVVLEMIREGKIAG RAVLIAGQPGTGKTAIAMGMAQALG PDTPFTAIAGSEIFSLEMSKTEALT QAFRRSIGVRIKEETEIIEG EVVEIQIDRPATGTGSKVGKLTLKT TEMETIYDLGTKMIESLTKDKVQAG DVITIDKATGKISKLGRSFT RARDYDAMGSQTKFVQCPDGELQKR KEVVHTVSLHEIDVINSRTQGFLAL FSGDTGEIKSEVREQINAKV AEWREEGKAEIIPGVLFIDEVHMLD IESFSFLNRALESDMAPVLIMATNR GITRIRGTSYQSPHGIPIDL LDRLLIVSTTPYSEKDTKQILRIRC EEEDVEMSEDAYTVLTRIGLETSLR YAIQLITAASLVCRKRKGTE
  VQVDDIKRVYSLFLDESRSTQYMKE YQDAFLFNELKGETMDTSTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors

Gene Information

• Gene Name: RUVBL2 RuvB like AAA ATPase 2 [ Homo sapiens (human) ]
• Official Symbol: RUVBL2
• Synonyms: RVB2; TIH2; ECP51; TIP48; CGI-46; ECP-51; INO80J; REPTIN; TIP49B; TAP54-beta
• Gene ID: 10856
• mRNA Refseq: NM_006666.3
• Protein Refseq: NP_006657.1
• MIM: 604788
• UniProt ID: Q9Y230