Recombinant Full Length Human SART3 Protein, C-Flag-tagged

• Cat.No.: SART3-1815HFL
• Product Overview: Recombinant Full Length Human SART3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 109.8 kDa
• AA Sequence: MATAAETSASEPEAESKAGPKADGE EDEVKAARTRRKVLSRAVAAATYKT MGPAWDQQEEGVSESDGDEY AMASSAESSPGEYEWEYDEEEEKNQ LEIERLEEQLSINVYDYNCHVDLIR LLRLEGELTKVRMARQKMSE IFPLTEELWLEWLHDEISMAQDGLD REHVYDLFEKAVKDYICPNIWLEYG QYSVGGIGQKGGLEKVRSVF ERALSSVGLHMTKGLALWEAYREFE SAIVEAARLEKVHSLFRRQLAIPLY DMEATFAEYEEWSEDPIPES VIQNYNKALQQLEKYKPYEEALLQA EAPRLAEYQAYIDFEMKIGDPARIQ LIFERALVENCLVPDLWIRY SQYLDRQLKVKDLVLSVHNRAIRNC PWTVALWSRYLLAMERHGVDHQVIS VTFEKALNAGFIQATDYVEI WQAYLDYLRRRVDFKQDSSKELEEL RAAFTRALEYLKQEVEERFNESGDP SCVIMQNWARIEARLCNNMQ KARELWDSIMTRGNAKYANMWLEYY NLERAHGDTQHCRKALHRAVQCTSD YPEHVCEVLLTMERTEGSLE DWDIAVQKTETRLARVNEQRMKAAE KEAALVQQEEEKAEQRKRARAEKKA LKKKKKIRGPEKRGADEDDE KEWGDDEEEQPSKRRRVENSIPAAG ETQNVEVAAGPAGKCAAVDVEPPSK QKEKAASLKRDMPKVLHDSS KDSITVFVSNLPYSMQEPDTKLRPL FEACGEVVQIRPIFSNRGDFRGYCY VEFKEEKSALQALEMDRKSV EGRPMFVSPCVDKSKNPDFKVFRYS TSLEKHKLFISGLPFSCTKEELEEI CKAHGTVKDLRLVTNRAGKP KGLAYVEYENESQASQAVMKMDGMT IKENIIKVAISNPPQRKVPEKPETR KAPGGPMLLPQTYGARGKGR TQLSLLPRALQRPSAAAPQAENGPA AAPAVAAPAATEAPKMSNADFAKLF LRK TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: SART3 spliceosome associated factor 3, U4/U6 recycling protein [ Homo sapiens (human) ]
• Official Symbol: SART3
• Synonyms: P100; p110; DSAP1; TIP110; p110(nrb); RP11-13G14
• Gene ID: 9733
• mRNA Refseq: NM_014706.4
• Protein Refseq: NP_055521.1
• MIM: 611684
• UniProt ID: Q15020