Recombinant Human SMAD4 protein, His-tagged

• Cat.No.: SMAD4-386H
• Product Overview: Recombinant Human SMAD4 protein(Met1-Asp552), fused with C-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-552 aa
• Form: Lyophilized from a 0.22 μm filtered solution of 20mM Tris-HCl, pH 8.0.
• Molecular Mass: 61.5 KDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 85 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: SMAD4 SMAD family member 4 [ Homo sapiens ]
• Official Symbol: SMAD4
• Synonyms: SMAD4; SMAD family member 4; MAD, mothers against decapentaplegic homolog 4 (Drosophila) , MADH4, SMAD, mothers against DPP homolog 4 (Drosophila); mothers against decapentaplegic homolog 4; DPC4; MAD homolog 4; SMAD, mothers against DPP homolog 4; deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; mothers against decapentaplegic, Drosophila, homolog of, 4; JIP; MADH4; MYHRS
• Gene ID: 4089
• mRNA Refseq: NM_005359
• Protein Refseq: NP_005350
• MIM: 600993
• UniProt ID: Q13485