Recombinant Full Length Human SMAD7 Protein, C-Flag-tagged

• Cat.No.: SMAD7-241HFL
• Product Overview: Recombinant Full Length Human SMAD7 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 46.2 kDa
• AA Sequence: MFRTKRSALVRRLWRSRAPGGEDEE EGAGGGGGGGELRGEGATDSRAHGA GGGGPGRAGCCLGKAVRGAK GHHHPHPPAAGAGAAGGAEADLKAL THSVLKKLKERQLELLLQAVESRGG TRTACLLLPGRLDCRLGPGA PAGAQPAQPPSSYSLPLLLCKVFRW PDLRHSSEVKRLCCCESYGKINPEL VCCNPHHLSRLCELESPPPP YSRYPMDFLKPTADCPDAVPSSAET GGTNYLAPGGLSDSQLLLEPGDRSH WCVVAYWEEKTRVGRLYCVQ EPSLDIFYDLPQGNGFCLGQLNSDN KSQLVQKVRSKIGCGIQLTREVDGV WVYNRSSYPIFIKSATLDNP DSRTLLVHKVFPGFSIKAFDYEKAY SLQRPNDHEFMQQPWTGFTVQISFV KGWGQCYTRQFISSCPCWLE
  VIFNSRTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway

Gene Information

• Gene Name: SMAD7 SMAD family member 7 [ Homo sapiens (human) ]
• Official Symbol: SMAD7
• Synonyms: CRCS3; MADH7; MADH8
• Gene ID: 4092
• mRNA Refseq: NM_005904.4
• Protein Refseq: NP_005895.1
• MIM: 602932
• UniProt ID: O15105