Recombinant Full Length Human SNAP23 Protein, C-Flag-tagged

• Cat.No.: SNAP23-841HFL
• Product Overview: Recombinant Full Length Human SNAP23 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Specificity of vesicular transport is regulated, in part, by the interaction of a vesicle-associated membrane protein termed synaptobrevin/VAMP with a target compartment membrane protein termed syntaxin. These proteins, together with SNAP25 (synaptosome-associated protein of 25 kDa), form a complex which serves as a binding site for the general membrane fusion machinery. Synaptobrevin/VAMP and syntaxin are believed to be involved in vesicular transport in most, if not all cells, while SNAP25 is present almost exclusively in the brain, suggesting that a ubiquitously expressed homolog of SNAP25 exists to facilitate transport vesicle/target membrane fusion in other tissues. The protein encoded by this gene is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 23.2 kDa
• AA Sequence: MDNLSSEEIQQRAHQITDESLESTR RILGLAIESQDAGIKTITMLDEQKE QLNRIEEGLDQINKDMRETE KTLTELNKCCGLCVCPCNRTKNFES GKAYKTTWGDGGENSPCNVVSKQPG PVTNGQLQQPTTGAASGGYI KRITNDAREDEMEENLTQVGSILGN LKDMALNIGNEIDAQNPQIKRITDK ADTNRDRIDIANARAKKLID
  STRTRPLEQKLISEEDLAANDILDY KDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport

Gene Information

• Gene Name: SNAP23 synaptosome associated protein 23 [ Homo sapiens (human) ]
• Official Symbol: SNAP23
• Synonyms: SNAP-23; SNAP23A; SNAP23B; HsT17016
• Gene ID: 8773
• mRNA Refseq: NM_003825.4
• Protein Refseq: NP_003816.2
• MIM: 602534
• UniProt ID: O00161