Recombinant Full Length Human SPDEF Protein, C-Flag-tagged

• Cat.No.: SPDEF-1429HFL
• Product Overview: Recombinant Full Length Human SPDEF Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.3 kDa
• AA Sequence: MGSASPGLSSVSPSHLLLPPDTVSR TGLEKAAAGAVGLERRDWSPSPPAT PEQGLSAFYLSYFDMLYPED SSWAAKAPGASSREEPPEEPEQCPV IDSQAPAGSLDLVPGGLTLEEHSLE QVQSMVVGEVLKDIETACKL LNITADPMDWSPSNVQKWLLWTEHQ YRLPPMGKAFQELAGKELCAMSEEQ FRQRSPLGGDVLHAHLDIWK SAAWMKERTSPGAIHYCASTSEESW TDSEVDSSCSGQPIHLWQFLKELLL KPHSYGRFIRWLNKEKGIFK
  IEDSAQVARLWGIRKNRPAMNYDKL SRSIRQYYKKGIIRKPDISQRLVYQ FVHPITRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transcription Factors

Gene Information

• Gene Name: SPDEF SAM pointed domain containing ETS transcription factor [ Homo sapiens (human) ]
• Official Symbol: SPDEF
• Synonyms: PDEF; bA375E1.3
• Gene ID: 25803
• mRNA Refseq: NM_012391.3
• Protein Refseq: NP_036523.1
• MIM: 608144
• UniProt ID: O95238