Recombinant Full Length Human STIM1 Protein, C-Flag-tagged

• Cat.No.: STIM1-1088HFL
• Product Overview: Recombinant Full Length Human STIM1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 74.8 kDa
• AA Sequence: MDVCVRLALWLLWGLLLHQGQSLSH SHSEKATGTSSGANSEESTAAEFCR IDKPLCHSEDEKLSFEAVRN IHKLMDDDANGDVDVEESDEFLRED LNYHDPTVKHSTFHGEDKLISVEDL WKAWKSSEVYNWTVDEVVQW LITYVELPQYEETFRKLQLSGHAMP RLAVTNTTMTGTVLKMTDRSHRQKL QLKALDTVLFGPPLLTRHNH LKDFMLVVSIVIGVGGCWFAYIQNR YSKEHMKKMMKDLEGLHRAEQSLHD LQERLHKAQEEHRTVEVEKV HLEKKLRDEINLAKQEAQRLKELRE GTENERSRQKYAEEELEQVREALRK AEKELESHSSWYAPEALQKW LQLTHEVEVQYYNIKKQNAEKQLLV AKEGAEKIKKKRNTLFGTFHVAHSS SLDDVDHKILTAKQALSEVT AALRERLHRWQQIEILCGFQIVNNP GIHSLVAALNIDPSWMGSTRPNPAH FIMTDDVDDMDEEIVSPLSM QSPSLQSSVRQRLTEPQHGLGSQRD LTHSDSESSLHMSDRQRVAPKPPQM SRAADEALNAMTSNGSHRLI EGVHPGSLVEKLPDSPALAKKALLA LNHGLDKAHSLMELSPSAPPGGSPH LDSSRSHSPSSPDPDTPSPV
  GDSRALQASRNTRIPHLAGKKAVAE EDNGSIGEETDSSPGRKKFPLKIFK KPLKKTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane

Gene Information

• Gene Name: STIM1 stromal interaction molecule 1 [ Homo sapiens (human) ]
• Official Symbol: STIM1
• Synonyms: GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
• Gene ID: 6786
• mRNA Refseq: NM_003156.4
• Protein Refseq: NP_003147.2
• MIM: 605921
• UniProt ID: Q13586