Recombinant Full Length Human TLR4 Protein, C-Flag-tagged

• Cat.No.: TLR4-614HFL
• Product Overview: Recombinant Full Length Human TLR4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. In silico studies have found a particularly strong binding of surface TLR4 with the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of Coronavirus disease-2019 (COVID-19). This receptor has also been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness, and with susceptibility to age-related macular degeneration. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 93.2 kDa
• AA Sequence: MSASRLAGTLIPAMAFLSCVRPESW EPCVEVVPNITYQCMELNFYKIPDN LPFSTKNLDLSFNPLRHLGS YSFFSFPELQVLDLSRCEIQTIEDG AYQSLSHLSTLILTGNPIQSLALGA FSGLSSLQKLVAVETNLASL ENFPIGHLKTLKELNVAHNLIQSFK LPEYFSNLTNLEHLDLSSNKIQSIY CTDLRVLHQMPLLNLSLDLS LNPMNFIQPGAFKEIRLHKLTLRNN FDSLNVMKTCIQGLAGLEVHRLVLG EFRNEGNLEKFDKSALEGLC NLTIEEFRLAYLDYYLDDIIDLFNC LTNVSSFSLVSVTIERVKDFSYNFG WQHLELVNCKFGQFPTLKLK SLKRLTFTSNKGGNAFSEVDLPSLE FLDLSRNGLSFKGCCSQSDFGTTSL KYLDLSFNGVITMSSNFLGL EQLEHLDFQHSNLKQMSEFSVFLSL RNLIYLDISHTHTRVAFNGIFNGLS SLEVLKMAGNSFQENFLPDI FTELRNLTFLDLSQCQLEQLSPTAF NSLSSLQVLNMSHNNFFSLDTFPYK CLNSLQVLDYSLNHIMTSKK QELQHFPSSLAFLNLTQNDFACTCE HQSFLQWIKDQRQLLVEVERMECAT PSDKQGMPVLSLNITCQMNK TIIGVSVLSVLVVSVVAVLVYKFYF HLMLLAGCIKYGRGENIYDAFVIYS SQDEDWVRNELVKNLEEGVP PFQLCLHYRDFIPGVAIAANIIHEG FHKSRKVIVVVSQHFIQSRWCIFEY EIAQTWQFLSSRAGIIFIVL QKVEKTLLRQQVELYRLLSRNTYLE WEDSVLGRHIFWRRLRKALLDGKSW NPEGTVGTGCNWQEATSITRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway

Gene Information

• Gene Name: TLR4 toll like receptor 4 [ Homo sapiens (human) ]
• Official Symbol: TLR4
• Synonyms: TOLL; CD284; TLR-4; ARMD10
• Gene ID: 7099
• mRNA Refseq: NM_138554.5
• Protein Refseq: NP_612564.1
• MIM: 603030
• UniProt ID: O00206