Recombinant Full Length Human UBE2C, His-tagged

• Cat.No.: UBE2C-221H
• Product Overview: Recombinant full length human UBE2C was expressed in E. coli cells using an N-terminal His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: UBE2C or ubiquitin-conjugating enzyme E2C is a member of the E2 ubiquitin-conjugating enzyme family which is required for the destruction of mitotic cyclins and for cell cycle progression. UBE2C acted as a dominant-negative inhibitor which blocks the destruction of mitotic cyclins A and B and the onset of anaphase in frog embryos and mammalian cells. High UBE2C mRNA expression is associated with poor disease-free survival and overall survival. High tumor grade, as well as high Ki67 protein expression, was more frequent in the high-expression group of UBE2C.
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~21 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl
• Full Length: Full L.

Gene Information

• Gene Name: UBE2C ubiquitin-conjugating enzyme E2C [ Homo sapiens ]
• Official Symbol: UBE2C
• Synonyms: UBE2C; ubiquitin-conjugating enzyme E2C; ubiquitin-conjugating enzyme E2 C; UBCH10; ubiquitin-protein ligase C; ubiquitin carrier protein C; ubiquitin carrier protein E2-C; cyclin-selective ubiquitin carrier protein; mitotic-specific ubiquitin-conjugating enzyme; dJ447F3.2
• Gene ID: 11065
• mRNA Refseq: NM_007019
• Protein Refseq: NP_008950
• MIM: 605574
• UniProt ID: O00762
• Chromosome Location: 20q13.12
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Cyclin B, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; protein binding; ubiquitin-protein ligase activity; contributes_to ubiquitin-protein ligase activity