Recombinant Full Length Human WNT5A Protein, C-Flag-tagged

• Cat.No.: WNT5A-195HFL
• Product Overview: Recombinant Full Length Human WNT5A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 42.2 kDa
• AA Sequence: MKKSIGILSPGVALGMAGSAMSSKF FLVALAIFFSFAQVVIEANSWWSLG MNNPVQMSEVYIIGAQPLCS QLAGLSQGQKKLCHLYQDHMQYIGE GAKTGIKECQYQFRHRRWNCSTVDN TSVFGRVMQIGSRETAFTYA VSAAGVVNAMSRACREGELSTCGCS RAARPKDLPRDWLWGGCGDNIDYGY RFAKEFVDARERERIHAKGS YESARILMNLHNNEAGRRTVYNLAD VACKCHGVSGSCSLKTCWLQLADFR KVGDALKEKYDSAAAMRLNS RGKLVQVNSRFNSPTTQDLVYIDPS PDYCVRNESTGSLGTQGRLCNKTSE GMDGCELMCCGRGYDQFKTV
  QTERCHCKFHWCCYVKCKKCTEIVD QFVCKTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway

Gene Information

• Gene Name: WNT5A Wnt family member 5A [ Homo sapiens (human) ]
• Official Symbol: WNT5A
• Synonyms: hWNT5A
• Gene ID: 7474
• mRNA Refseq: NM_003392.7
• Protein Refseq: NP_003383.4
• MIM: 164975
• UniProt ID: P41221

Reviews

11/14/2019

Satisfied with performance, quality.

08/27/2019

Great product, satisfied customer.

02/27/2019

High purity, works well.

Q&As

What role does WNT5A play in cancer biology?

Its role in cancer is complex, acting as a tumor suppressor or promoter depending on context.

How does WNT5A interact with its receptors to initiate signal transduction?

It binds to Frizzled receptors and co-receptors, activating non-canonical Wnt signaling pathways.

In which developmental processes is WNT5A critically involved?

WNT5A is involved in embryonic development, particularly in limb formation and organogenesis.

How is WNT5A implicated in immune system regulation?

WNT5A modulates various immune responses, influencing inflammation and cell migration.

What is the primary role of WNT5A in cellular processes?

WNT5A is crucial in regulating cell proliferation, migration, and differentiation.

What therapeutic strategies are being explored involving WNT5A modulation?

Strategies include developing WNT5A modulators for potential use in cancer therapy and regenerative medicine.

Are there known genetic mutations in WNT5A associated with specific diseases?

Genetic mutations and altered expression of WNT5A are linked to developmental disorders and cancer.