Recombinant Full Length Human WNT5A Protein, C-Flag-tagged

• Cat.No.: WNT5A-195HFL
• Product Overview: Recombinant Full Length Human WNT5A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 42.2 kDa
• AA Sequence: MKKSIGILSPGVALGMAGSAMSSKF FLVALAIFFSFAQVVIEANSWWSLG MNNPVQMSEVYIIGAQPLCS QLAGLSQGQKKLCHLYQDHMQYIGE GAKTGIKECQYQFRHRRWNCSTVDN TSVFGRVMQIGSRETAFTYA VSAAGVVNAMSRACREGELSTCGCS RAARPKDLPRDWLWGGCGDNIDYGY RFAKEFVDARERERIHAKGS YESARILMNLHNNEAGRRTVYNLAD VACKCHGVSGSCSLKTCWLQLADFR KVGDALKEKYDSAAAMRLNS RGKLVQVNSRFNSPTTQDLVYIDPS PDYCVRNESTGSLGTQGRLCNKTSE GMDGCELMCCGRGYDQFKTV
  QTERCHCKFHWCCYVKCKKCTEIVD QFVCKTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway

Gene Information

• Gene Name: WNT5A Wnt family member 5A [ Homo sapiens (human) ]
• Official Symbol: WNT5A
• Synonyms: hWNT5A
• Gene ID: 7474
• mRNA Refseq: NM_003392.7
• Protein Refseq: NP_003383.4
• MIM: 164975
• UniProt ID: P41221