Recombinant Mouse Wnt5a protein, GST-tagged

• Cat.No.: Wnt5a-1492M
• Product Overview: Recombinant Mouse Wnt5a aa. (Ile62~Lys380) fused with N-terminal GST tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: GST
• Form: Freeze-dried powder
• Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ile62~Lys380
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Isoelectric Point: 8.8

Gene Information

• Gene Name: Wnt5a wingless-type MMTV integration site family, member 5A [ Mus musculus (house mouse) ]
• Official Symbol: Wnt5a
• Synonyms: Wnt5a; wingless-type MMTV integration site family, member 5A; Wnt-5a; 8030457G12Rik; protein Wnt-5a; wingless-related MMTV integration site 5A
• Gene ID: 22418
• mRNA Refseq: NM_001256224.1
• Protein Refseq: NP_001243153.1
• UniProt ID: P22725

Reviews

11/14/2019

Satisfied with performance, quality.

08/27/2019

Great product, satisfied customer.

02/27/2019

High purity, works well.

Q&As

What role does WNT5A play in cancer biology?

Its role in cancer is complex, acting as a tumor suppressor or promoter depending on context.

How does WNT5A interact with its receptors to initiate signal transduction?

It binds to Frizzled receptors and co-receptors, activating non-canonical Wnt signaling pathways.

In which developmental processes is WNT5A critically involved?

WNT5A is involved in embryonic development, particularly in limb formation and organogenesis.

How is WNT5A implicated in immune system regulation?

WNT5A modulates various immune responses, influencing inflammation and cell migration.

What is the primary role of WNT5A in cellular processes?

WNT5A is crucial in regulating cell proliferation, migration, and differentiation.

What therapeutic strategies are being explored involving WNT5A modulation?

Strategies include developing WNT5A modulators for potential use in cancer therapy and regenerative medicine.

Are there known genetic mutations in WNT5A associated with specific diseases?

Genetic mutations and altered expression of WNT5A are linked to developmental disorders and cancer.