Recombinant Full Length Thermoanaerobacter Thermosulfurogenes Probable Starch Degradation Products Transport System Permease Protein Amyc(Amyc) Protein, His-Tagged

• Cat.No.: RFL31248TF
• Product Overview: Recombinant Full Length Thermoanaerobacter thermosulfurogenes Probable starch degradation products transport system permease protein AmyC(amyC) Protein (P37729) (1-274aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Thermoanaerobacterium thermosulfurigenes (Clostridium thermosulfurogenes)
• Tag: His
• Form: Lyophilized powder
• Protein length: Full Length (1-274)
• AA Sequence: MRKVHVQKYLLTFLGIVLSLLWISP FYIILVNSFKTKLELFTNTLSLPKS LMLDNYKTAA ANLNLSEAFSNTLIITVFSILIIAI FSSMTAYALQRVKRKSSVIIYMIFT VAMLIPFQSV MIPLVAEFGKFHFLTRSGLVFMYLG FGSSLGVFLYYGALKGIPTSLDEAA LIDGCSRFRI YWNIILPLLNPTTITLAVLDIMWIW NDYLLPSLVINKVGSRTLPLMIFYF FSQYTKQWNL GMAGLTIAILPVVIFYFLAQRKLVT AIIAGAVKQ
• Purity: Greater than 90% as determined by SDS-PAGE.
• Applications: SDS-PAGE
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: amyC
• Synonyms: amyC; Probable starch degradation products transport system permease protein AmyC
• UniProt ID: P37729

Gene Information

• Gene Name: amyC
• Synonyms: amyC; Probable starch degradation products transport system permease protein AmyC
• UniProt ID: P37729

Reviews

07/21/2021

Their ability to accommodate the requirements of large-scale experiments and guarantee a reliable supply streamlines my research operations, eliminating any concerns related to potential shortages.

07/19/2021

the manufacturer's supply management capabilities assure a seamless and continuous provision of the AMY2B protein.

10/26/2019

I am confident that the protein will reliably perform in my assays, providing accurate and reproducible results.

03/20/2019

Their team of experts is readily available to address any queries or concerns I may have regarding the AMY2B protein.

Q&As

Can the AMYC protein be used as a biomarker for certain health conditions?

The AMYC protein has been explored as a potential biomarker for various health conditions due to its involvement in carbohydrate metabolism. For example, salivary amylase levels have been suggested as a biomarker for stress response and psychological or physiological stress.

How is the AMYC protein measured or quantified in research or clinical settings?

The levels of the AMYC protein can be measured by analyzing saliva samples. Enzymatic assays, such as the colorimetric or spectrophotometric method, can be used to measure the activity of salivary amylase. This involves measuring the rate of starch hydrolysis by the enzyme in the presence of a specific substrate. More recently, advancements in technology have led to the development of rapid point-of-care devices that can directly measure salivary amylase levels on-site, providing a convenient and non-invasive method for assessment.

How is the AMYC protein involved in carbohydrate digestion?

The AMYC protein, also known as salivary amylase, plays a crucial role in the digestion of carbohydrates. It is primarily produced in the salivary glands and secreted into the oral cavity during the process of chewing and saliva production.

Does the AMYC protein have any non-digestive functions?

While the primary function of the AMYC protein is associated with digestion, recent research suggests that it may have non-digestive roles as well. Some studies have indicated that salivary amylase levels might be associated with taste perception and food preferences. Additionally, the AMYC protein has been linked to immune system regulation, as it can interact with certain pathogens or defense mechanisms in the oral cavity. However, further research is needed to fully understand these potential non-digestive functions.

Are there any diseases or conditions associated with alterations in AMYC protein levels or activity?

Altered levels or activity of the AMYC protein have been implicated in various diseases and conditions. For example, increased salivary amylase levels have been associated with conditions such as obesity, metabolic syndrome, and insulin resistance. Conversely, reduced AMYC protein levels or amylase deficiency conditions have been linked to certain metabolic disorders, such as glycogen storage diseases. However, the exact mechanisms and clinical implications of these associations are still being studied.

Can variations in the AMYC gene or levels of the AMYC protein be linked to certain health conditions or diseases?

There is ongoing research exploring the potential links between variations in the AMY1A gene and certain health conditions or diseases. Some studies have suggested that individuals with a higher number of copies of the AMY1A gene (and therefore higher levels of salivary amylase) may have a lower risk of developing obesity and related metabolic disorders, such as type 2 diabetes. However, more research is needed to establish a clear cause-and-effect relationship and determine the underlying mechanisms.

Are there any potential implications for personalized nutrition or dietary recommendations based on variations in the AMYC protein?

Variations in the AMYC protein can potentially inform personalized nutrition and dietary recommendations. Research has shown that individuals with a higher number of copies of the AMY1A gene may have a better ability to digest and metabolize carbohydrates. Therefore, they may benefit from diets that include a higher proportion of carbohydrates.

How is the AMYC gene and protein regulated?

The regulation of the AMYC gene and protein involves various factors and mechanisms. Transcription factors, which are proteins that bind to specific DNA sequences, play a crucial role in controlling the expression of the AMYC gene. These transcription factors can either enhance or inhibit the production of the AMYC mRNA, which is then translated into the AMYC protein.

Are there any genetic variations or polymorphisms associated with the AMYC protein?

Yes, genetic variations or polymorphisms can influence AMYC protein levels and enzymatic activity. Certain single nucleotide polymorphisms (SNPs) in the AMY1A gene, which encodes the AMYC protein, have been associated with differences in salivary amylase levels and activity. These genetic variations can affect an individual's ability to efficiently breakdown carbohydrates and may have implications for metabolic health.

What are the limitations or challenges associated with studying the AMYC protein or variations in the AMYC gene?

Studying the AMYC protein and variations in the AMYC gene has its challenges and limitations. Firstly, variations in the AMY1A gene and salivary amylase levels can differ among populations, making it challenging to establish consistent associations across diverse populations. Additionally, other factors such as diet, lifestyle, and the complexity of the human digestive system can complicate the study of the AMYC protein's role in health and disease.

Can the AMYC protein be found in other tissues or organs besides the salivary glands?

While the production of the AMYC protein is most prominent in the salivary glands, low levels of AMYC protein expression have been observed in other tissues and organs as well. These include the pancreas, liver, testis, and fallopian tubes. However, the physiological significance of AMYC protein expression in these tissues is not yet fully understood.

Is there any potential for therapies or interventions targeting the AMYC protein for conditions related to carbohydrate metabolism?

While there are currently no therapies specifically targeting the AMYC protein, further understanding of its role in carbohydrate metabolism may lead to potential therapeutic interventions in the future. Developing drugs or interventions that modulate the activity or levels of salivary amylase could potentially be explored to optimize carbohydrate digestion and metabolism in individuals with specific health conditions or metabolic disorders.

What factors can affect the levels or activity of the AMYC protein?

Several factors can influence the levels or activity of the AMYC protein. One important factor is genetic variation, as mentioned earlier. Individuals may have different numbers of copies of the AMY1A gene, which can impact the levels of salivary amylase produced. Environmental factors, such as diet and stress, can also influence the levels of the AMYC protein. For instance, a high-carbohydrate diet can increase salivary amylase levels, while chronic stress can lead to decreased levels. Hormones, medications, and certain medical conditions may also affect the AMYC protein.

Are there any potential therapeutic applications or interventions related to the AMYC protein?

While there are no specific therapeutic interventions directly targeting the AMYC protein at present, research on the AMYC protein and its association with various health conditions may have implications for personalized medicine and dietary recommendations. Understanding an individual's salivary amylase levels or genetic variations in the AMYC gene may help in tailoring diets or lifestyle interventions for optimal carbohydrate metabolism.