Recombinant Gaussia Luciferase Protein, Streptavidin-Labled Luciferase-105G

Recombinant Gaussia Luciferase Protein, Streptavidin-Labled

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Cat.No. : Luciferase-105G Optional Service: Optional requirements on this protein
Product Overview : Gaussia(M2)-Luciferase-Streptavidin is a conjugate of one of the brightest luciferases and a protein with the highest known affinity in nature.
in vitro fusion of two independently expressed proteins in different organisms Gaussia(M2)-Avitag-biotin-Streptavidin
Description : Gaussia Luciferase is a new reporter gene isolated from the marine copepod Gaussia princeps.
Form : Clear liquid
Bio-activity : Recommended CTZ concentration for luminometer: 50 μM
Around 3-fold brighter than wildtyp GLuc-SA
Binding efficiency greater than 90%
Very sensitive: use 0.1 to 1 ng of GLuc-SA for biotin coated plates
Applications : This fusion protein can be used to detect trace amounts of biotinylated protein, DNA, or other biotinylated bio-molecules.
Storage : Store protein at -80 centigrade for maximum shelf-life, avoid repeated freezing
Concentration : 1 mg/mL
Storage Buffer : TBS, pH 7.8 + nonproteinogenic stabilizers
Dilutions : TBS or Gaussia dilution buffer
Official Symbol : Luciferase
Synonyms : Luciferase; Gaussia luciferase; GLuc
Dilution series (1:2) of GLuc-SA in a black 96-well plate :

Picture was taken with a regular camera.

Representative SDS PAGE of GLuc-SA in native and reduced and oxidized form :

One GLuc-SA molecule consists of one GLucAvitag protein (27 kDa) and 4 Streptavidin subunits (13 kDa).

Biotin-Agarose beads (100 μm) coated with SA-GLuc examined with Nikon microscope with no filter in total darkness. :

Biotin-Agarose beads (100 μm) coated with GLuc-SA examined with Olympus LV-200 bioluminescence imaging :

Biotin coated plate binding activity comparison :

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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