Active Recombinant Equine IL1A

• Cat.No.: IL1A-207E
• Product Overview: Recombinant Equine IL1A was expressed in Yeast.

Specification

• Description: The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
• Source: Yeast
• Species: Equine
• Form: Lyophilized.
• Bio-activity: In testing
• Molecular Mass: 18.1 kDa
• AA Sequence: SVHYNFQSNT KYNFMRIVNH QCTLNDALNQ SVIRDTSGQY LATAALNNLD DAVKFDMGAY TSEEDSQLPV TLRISKTRLF VSAQNEDEPV LLKEMPDTPK TIKDETNLLF FWERHGSKNY FKSVAHPKLF IATKQGKLVH MARGQPSITD FQILDNQF (158)
• Purity: >95% as visualized by SDS-PAGE analysis.
• Storage: Stable for up to twelve months from date of receipt at °C. Stable for at least 3 months when stored in working aliquots with a carrier protein at °C. Avoid repeated freeze/thaw cycles.
• Reconstitution: Reconstitute with sterile phosphate-buffered saline containing at least 0.1% carrier protein.
• Tag: Non

Gene Information

• Gene Name: IL1A interleukin 1, alpha [ Equus caballus (horse) ]
• Official Symbol: IL1A
• Synonyms: IL1A; interleukin 1, alpha; interleukin-1 alpha; IL-1 alpha
• Gene ID: 100064969
• mRNA Refseq: NM_001082500
• Protein Refseq: NP_001075969
• UniProt ID: Q28385
• Chromosome Location: 15
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Inflammatory bowel disease (IBD)

Reviews

04/08/2021

High solubility.

07/02/2020

Good for intracellular staining.

03/13/2019

Effective in apoptosis assays.

Q&As

What methods are employed to dissect the intricate cross-talk between IL-1α and other cytokines, shedding light on its role in immune modulation?

Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.

What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts?

IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.

How are cutting-edge techniques like single-cell RNA sequencing utilized to unravel the heterogeneity in IL-1α-responsive cell populations and their functional implications?

Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.

How is the intricate web of IL-1α's pro-inflammatory signaling pathways decoded using advanced experimental techniques?

IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.

How are state-of-the-art genetic editing tools such as CRISPR-Cas9 applied to unravel IL-1α's precise contributions to inflammatory diseases?

Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.

How do researchers manipulate in vitro co-culture systems to dissect the dynamic interplay between immune cells and stromal cells in IL-1α-mediated responses?

In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.

What experimental strategies enable the identification of specific cellular receptors and downstream effectors responsible for IL-1α's diverse physiological effects?

Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.

How are sophisticated imaging techniques like live-cell microscopy harnessed to capture the real-time dynamics of IL-1α release and its impact on neighboring cells?

Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.

How is the quantitative relationship between IL-1α levels and disease progression established through longitudinal studies and statistical modeling?

Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.

How do multi-omics analyses, including transcriptomics and proteomics, provide a holistic view of the network of molecules influenced by IL-1α signaling?

Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.