Recombinant Guinea pig Il4 Protein, His-tagged

• Cat.No.: Il4-2318G
• Product Overview: Recombinant Guinea pig Il4 Protein (Cys25-Arg144) with a N-His tag was expressed in 293F.

Specification

• Description: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
• Source: HEK293F
• Species: Guinea pig
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 15.3 kDa
  Accurate Molecular Mass: \
• Protein length: Cys25-Arg144
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Il4 interleukin 4 [ Cavia porcellus (domestic guinea pig) ]
• Official Symbol: Il4
• Synonyms: IL4; interleukin 4; IL-4; BSF-1; interleukin-4
  B-cell stimulatory factor 1
  lymphocyte stimulatory factor 1
• Gene ID: 100720403
• mRNA Refseq: NM_001257263
• Protein Refseq: NP_001244192
• UniProt ID: G0WM66