Recombinant HIV-1 Bal TAT Protein

• Cat.No.: HIV1BalTAT-118H

Specification

• Species: HIV
• Source: E.coli
• Tag: Non
• Description: Full length (101 amino acids) Recombinant HIV-1 Bal tat produced in the E. coli Expression System. This protein binds to murine monoclonal antibodies of defined epitope specificity and rabbit and human serum polyclonal antibodies in ELISA and Western ELISA.
• Form: Frozen Liquid
• Molecular Mass: 16 kD
• Purity: > 95 % by SDS-PAGE
• Applications: ELISA;
  Western ELISA;
  Anti-tat Drug Screening;
  Immunization;
  Transcriptional Activation.
• Notes: Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dilute tat stock solution in Tris- saline or salins-phosphate buffer, pH 7-8, immediately before use. Tat readily oxidizes in buffer solutions which may change its LTR- dependent transcriptional activation activity.
  Transcriptional activation assays with tat are performed in 1-10µg/ml range. ELISA and Western ELISA require tat in 10-100ng protein range.
• Stability: At least 6 months at -75 centigrade.
• Storage: Store at -75 centigrade.
• Dilutions: 50mM Tris, 0.1M NaCl, 2mM dtt, pH7.8