Recombinant Human A2ML1 protein, His & T7-tagged A2ML1-5676H

Recombinant Human A2ML1 protein, His & T7-tagged

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Recombinant Human A2ML1 protein, His & T7-tagged

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Cat.No. : A2ML1-5676H
Product Overview : Recombinant Human A2ML1 aa. (Gln1119~Thr1348 (Accession # A8K2U0)) fused with N-terminal His & T7 tag was produced in E. coli cells.
Description : This gene encodes a member of the alpha-macroglobulin superfamily. The encoded protein is thought to be an N-glycosylated monomeric protein that acts as an inhibitor of several proteases. It has been shown to form covalent interactions with proteases, and has been reported as the p170 antigen recognized by autoantibodies in the autoimmune disease paraneoplastic pemphigus (PNP; PMID:20805888). Mutations in these gene have also been associated with some cases of Noonan syndrome (NS; PMID:24939586) as well as some cases of otitis media (PMID:26121085). Alternative splicing results in multiple transcript variants encoding different isoforms.
Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 28.8kDa
Protein length : Gln1119~Thr1348 (Accession # A8K2U0)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 8.9.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
SDS-PAGE :

Gene Name : A2ML1 alpha-2-macroglobulin like 1 [ Homo sapiens (human) ]
Official Symbol : A2ML1
Synonyms : A2ML1; alpha-2-macroglobulin like 1; p170; CPAMD9; alpha-2-macroglobulin-like protein 1; C3 and PZP-like, alpha-2-macroglobulin domain containing 9
Gene ID : 144568
mRNA Refseq : NM_001282424.2
Protein Refseq : NP_001269353.1
UniProt ID : A8K2U0

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