Recombinant Human ACE, His-tagged

• Cat.No.: ACE-744H
• Product Overview: Recombinant human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag sequence and expressed inNSO cells. By SDS-PAGE, the apparent molecular weight of the glycosylated protein appears larger to be than 120 kDa under both reducing and non-reducing conditions.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 30-1261 a.a.
• Description: ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. ACE gene polymorphism has been a recent topic in Alzheimer"s disease studies. ACE is a type I membrane protein and exists in two isoforms, somatic and germinal.
• Purity: ≥95% by SDS-PAGE.
• Form: Liquid.In 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl2, 40% glycerol, pH 7.5. Please refer to vial label for lot-specific concentration.
• Endotoxins: ≤1.0 EU/µg ACE.
• Specific Activity: ≥1 unit/µg protein.
• Unit Definition: One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
• Toxicity: Standard Handling(MSDS available upon request).
• Storage: ≤ -70℃. Avoid freeze/thaw. Following initial thaw, aliquot and freeze (-70°C).

Gene Information

• Gene Name: ACE angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 [ Homo sapiens ]
• Synonyms: ACE; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; DCP; ACE1; DCP1; CD143; MVCD3; MGC26566; angiotensin-converting enzyme; kininase II; peptidase P; CD143 antigentesticular ECA; carboxycathepsin; dipeptidyl carboxypeptidase 1; dipeptidyl carboxypeptidase I; angiotensin converting enzyme, somatic isoform; EC 3.4.15.1; Angiotensin-converting enzyme, soluble form
• Gene ID: 1636
• mRNA Refseq: NM_000789
• Protein Refseq: NP_000780
• MIM: 106180
• UniProt ID: P12821
• Chromosome Location: 17q23.3
• Pathway: Chagas disease; Hypertrophic cardiomyopathy (HCM); Renin-angiotensin system
• Function: actin binding; bradykinin receptor binding; carboxypeptidase activity; chloride ion binding; drug binding; metal ion binding; metallopeptidase activity; peptidase activity; peptide binding; peptidyl-dipeptidase activity; protein binding; zinc ion binding