Active Recombinant Human ACE Protein, His-tagged

• Cat.No.: ACE-011H
• Product Overview: Recombinant Human ACE Protein (30-1256aa) with His tag was expressed in insect cells.

Specification

• Description: This gene encodes an enzyme involved in in blood pressure regulation and electrolyte balance. It catalyzes the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. ACE also inactivates the vasodilator protein, bradykinin. Accordingly, the encoded enzyme increases blood pressure and is a drug target of ACE inhibitors, which are often prescribed to reduce blood pressure. This enzyme additionally plays a role in fertility through its ability to cleave and release GPI-anchored proteins in testes. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme. This polymorphism, as well as mutations in this gene, have been implicated in a wide variety of diseases including cardiovascular pathophysiologies, psoriasis, renal disease, stroke, and Alzheimer's disease. Alternative splicing results in multiple transcript variants.
• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 1000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPPGFSAFK(Dnp)-OH per minute at 25 centigrade.
• Molecular Mass: 142kDa(1235aa)
• Protein length: 30-1256aa
• AA Sequence: LDPGLQPGNFSADEAGAQLFAQSYN SSAEQVLFQSVAASWAHDTNITAEN ARRQEEAALLSQEFAEAWGQKAKEL YEPIWQNFTDPQLRRIIGAVRTLGS ANLPLAKRQQYNALLSNMSRIYSTA KVCLPNKTATCWSLDPDLTNILASS RSYAMLLFAWEGWHNAAGIPLKPLY EDFTALSNEAYKQDGFTDTGAYWRS WYNSPTFEDDLEHLYQQLEPLYLNL HAFVRRALHRRYGDRYINLRGPIPA HLLGDMWAQSWENIYDMVVPFPDKP NLDVTSTMLQQGWNATHMFRVAEEF FTSLELSPMPPEFWEGSMLEKPADG REVVCHASAWDFYNRKDFRIKQCTR VTMDQLSTVHHEMGHIQYYLQYKDL PVSLRRGANPGFHEAIGDVLALSVS TPEHLHKIGLLDRVTNDTESDINYL LKMALEKIAFLPFGYLVDQWRWGVF SGRTPPSRYNFDWWYLRTKYQGICP PVTRNETHFDAGAKFHVPNVTPYIR YFVSFVLQFQFHEALCKEAGYEGPL HQCDIYRSTKAGAKLRKVLQAGSSR PWQEVLKDMVGLDALDAQPLLKYFQ PVTQWLQEQNQQNGEVLGWPEYQWH PPLPDNYPEGIDLVTDEAEASKFVE EYDRTSQVVWNEYAEANWNYNTNIT TETSKILLQKNMQIANHTLKYGTQA RKFDVNQLQNTTIKRIIKKVQDLER AALPAQELEEYNKILLDMETTYSVA TVCHPNGSCLQLEPDLTNVMATSRK YEDLLWAWEGWRDKAGRAILQFYPK YVELINQAARLNGYVDAGDSWRSMY ETPSLEQDLERLFQELQPLYLNLHA YVRRALHRHYGAQHINLEGPIPAHL LGNMWAQTWSNIYDLVVPFPSAPSM DTTEAMLKQGWTPRRMFKEADDFFT SLGLLPVPPEFWNKSMLEKPTDGRE VVCHASAWDFYNGKDFRIKQCTTVN LEDLVVAHHEMGHIQYFMQYKDLPV ALREGANPGFHEAIGDVLALSVSTP KHLHSLNLLSSEGGSDEHDINFLMK MALDKIAFIPFSYLVDQWRWRVFDG SITKENYNQEWWSLRLKYQGLCPPV PRTQGDFDPGAKFHIPSSVPYIRYF VSFIIQFQFHEALCQAAGHTGPLHK CDIYQSKEAGQRLATAMKLGFSRPW PEAMQLITGQPNMSASAMLSYFKPL LDWLRTENELHGEKLGWPQYNWTPN SARSEGPLPDSGRVSFLGLDLDAQQ AR
• Endotoxin: < 1 EU per 1 µg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: ACE angiotensin I converting enzyme [ Homo sapiens (human) ]
• Official Symbol: ACE
• Synonyms: ACE; angiotensin I converting enzyme; DCP; ACE1; DCP1; CD143; angiotensin-converting enzyme; CD143 antigen; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; angiotensin converting enzyme, somatic isoform; carboxycathepsin; dipeptidyl carboxypeptidase 1; dipeptidyl carboxypeptidase I; kininase II; peptidase P; EC 3.4.15.1
• Gene ID: 1636
• mRNA Refseq: NM_000789
• Protein Refseq: NP_000780
• MIM: 106180
• UniProt ID: P12821