Recombinant Human ACTA1 protein, GST-tagged

• Cat.No.: ACTA1 -125H
• Product Overview: Recombinant Human ACTA1(1 a.a. - 377 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.

Specification

• Description: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 68.5 kDa
• AA Sequence: MCDEDETTALVCDNGSGLVKAGFAG DDAPRAVFPSIVGRPRHQGVMVGMG QKDSYVGDEAQSKRGILTLKYPIEH GIITNWDDMEKIWHHTFYNELRVAP EEHPTLLTEAPLNPKANREKMTQIM FETFNVPAMYVAIQAVLSLYASGRT TGIVLDSGDGVTHNVPIYEGYALPH AIMRLDLAGRDLTDYLMKILTERGY SFVTTAEREIVRDIKEKLCYVALDF ENEMATAASSSSLEKSYELPDGQVI TIGNERFRCPETLFQPSFIGMESAG IHETTYNSIMKCDIDIRKDLYANNV MSGGTTMYPGIADRMQKEITALAPS TMKIKIIAPPERKYSVWIGGSILAS LSTFQQMWITKQEYDEAGPSIVHRK CF
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: ACTA1 actin, alpha 1, skeletal muscle [ Homo sapiens ]
• Official Symbol: ACTA1
• Synonyms: ACTA1; actin, alpha 1, skeletal muscle; ACTA; actin, alpha skeletal muscle; alpha-actin-1; alpha skeletal muscle actin; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM
• Gene ID: 58
• mRNA Refseq: NM_001100
• Protein Refseq: NP_001091
• MIM: 102610
• UniProt ID: P68133
• Chromosome Location: 1q42.13
• Pathway: Caspase cascade in apoptosis, organism-specific biosystem; Hypothetical Network for Drug Addiction, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; RhoA signaling pathway, organism-specific biosystem; Signaling events mediated by focal adhesion kinase, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem
• Function: ADP binding; ATP binding; myosin binding; nucleotide binding; protein binding; structural constituent of cytoskeleton