"ACTA1" Related Products

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Recombinant Human ACTA1 protein, GST-tagged

Cat.No.: ACTA1-125H
Product Overview: Recombinant Human ACTA1(1 a.a. - 377 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ.
Description: The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
Source: Wheat Germ
Species: Human
Tag: GST
Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Molecular Mass: 68.5 kDa
AA Sequence: MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEH GIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRT TGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDF ENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV MSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRK CF
Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
Notes: Best use within three months from the date of receipt of this protein.
Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Gene Name: ACTA1 actin, alpha 1, skeletal muscle [ Homo sapiens ]
Official Symbol: ACTA1
Synonyms: ACTA1; actin, alpha 1, skeletal muscle; ACTA; actin, alpha skeletal muscle; alpha-actin-1; alpha skeletal muscle actin; ASMA; CFTD; MPFD; NEM1; NEM2; NEM3; CFTD1; CFTDM;
Gene ID: 58
mRNA Refseq: NM_001100
Protein Refseq: NP_001091
MIM: 102610
UniProt ID: P68133
Chromosome Location: 1q42.13
Pathway: Caspase cascade in apoptosis, organism-specific biosystem; Hypothetical Network for Drug Addiction, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; RhoA signaling pathway, organism-specific biosystem; Signaling events mediated by focal adhesion kinase, organism-specific biosystem; Striated Muscle Contraction, organism-specific biosystem;
Function: ADP binding; ATP binding; myosin binding; nucleotide binding; protein binding; structural constituent of cytoskeleton;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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