| Description : |
This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
43.25 kDa |
| AA Sequence : |
MVVERDDGSTLMEIDGDKGKQGGPT YYIDTNALRVPRENMEAISPLKNGM VEDWDSFQAILDHTYKMHVKSEASL HPVLMSEAPWNTRAKREKLTELMFE HYNIPAFFLCKTAVLTAFANGRSTG LILDSGATHTTAIPVHDGYVLQQGI VKSPLAGDFITMQCRELFQEMNIEL VPPYMIATKEAVREGSPANWKRKEK LPQVTRSWHNYMCNCVIQDFQASVL QVSDSTYDEQVAAQMPTVHYEFPNG YNCDFGAERLKIPEGLFDPSNVKGL SGNTMLGVSHVVTTSVGMCDIDIRP GLYGSVIVAGGNTLIQSFTDRLNRE LSQKTPPSMRLKLIANNTTVERRFS SWIGGSILASLGTFQQMWISKQEYE EGGKQCVERKCPTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
