Human ACTL6A Knockdown Cell Lysate

• Cat.No.: ACTL6A-024HKCL
• Product Overview: WB-validated ACTL6A Knockdown HeLa Cell Lysate

Specification

• Species: Human
• Source: HeLa
• Tag: Non
• Description: This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described.
• Form: Cell-Tissue Lysis buffer
• Molecular Mass: 47 kDa
• Notes: Instruction of use: This knockdown cell lysate should be paired with wild-type HeLa cell lysate for use. For Western blotting, we recommend running wild-type and knockdown lysates on the same gel, and loading each well with equal volume and equal amount of total proteins.
• Storage: Store at -20 centigrade for two years.
• Concentration: Lot-specific
• Shipping: Blue Ice
• Components: 1 vial of 100 μg WT HeLa cell lysate 1 vial of 100 μg ACTL6A KD HeLa cell lysate
• Protein Families: Druggable Genome, Transcription Factors
• Lysate QC: RT-qPCR; Western Blotting (WB)

Gene Information

• Gene Name: ACTL6A actin-like 6A [ Homo sapiens (human) ]
• Official Symbol: ACTL6A
• Synonyms: ACTL6A; actin-like 6A; actin-like protein 6A; actin related protein 4; Actl6; Arp4; BAF complex 53 kDa subunit; BAF53A; Baf53a; BRG1 associated factor; INO80 complex subunit K; INO80K; BAF53; arpNbeta; hArpN beta; actin-related protein 4; BRG1-associated factor 53A; actin-related protein Baf53a; 53 kDa BRG1-associated factor A; ACTL6; ARPN-BETA
• Gene ID: 86
• mRNA Refseq: NM_004301
• Protein Refseq: NP_004292
• MIM: 604958
• UniProt ID: O96019