Recombinant Human ADAM10 protein, His-tagged

• Cat.No.: ADAM10-7638H
• Product Overview: Recombinant Human ADAM10 aa. (Arg420~Val641 (Accession # O14672)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Arg420~Val641
• Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.7kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ADAM10 ADAM metallopeptidase domain 10 [ Homo sapiens (human) ]
• Official Symbol: ADAM10
• Synonyms: RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717; a disintegrin and metalloprotease domain 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; disintegrin and metalloproteinase domain-containing protein 10
• Gene ID: 102
• mRNA Refseq: NM_001110.3
• Protein Refseq: NP_001101.1
• UniProt ID: O14672