Species : |
Human |
Source : |
HEK293 |
Tag : |
Fc |
Protein Length : |
Leu20-His925 |
Tag : |
C-hFc |
Form : |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human SEZ6, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-SEZ6 Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. |
Molecular Mass : |
The protein has a predicted MW of 124.53 kDa. Due to glycosylation, the protein migrates to 140-200 kDa based on Tris-Bis PAGE result. |
Endotoxin : |
Less than 1EU per μg by the LAL method. |
Purity : |
> 95% as determined by Tris-Bis PAGE |
Storage : |
Store at -20 to -80°C for 12 months as supplied from date of receipt; -80°C for 3-6 months after reconstitution; 2-8°C for 2-7 days after reconstitution; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Reconstitution : |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |