Recombinant Human ADGRE5 Protein, C-His-tagged

• Cat.No.: ADGRE5-217H
• Product Overview: Recombinant Human ADGRE5 Protein with C-His tag was expressed in E. coli.

Specification

• Description: CD97 is a member of the EGF-TM7 (seven-span transmembrane) protein family, which is characterized by an extended extracellular region with a variable number of N-terminal EGF-like domains coupled to a TM7 stalk. It is expressed by leukocytes following activation. CD97 binds to its cellular ligand CD55 (decay accelerating factor) and protects several cell types from complement-mediated damage. The CD97-CD55 interaction may play a role in cellular activation, migration and adhesion following inflammation. CD97 expression is increased in thyroid cancer, paralleling dedifferentiation and tumor staging in this disease. Many colorectal cell lines are also CD97+, with CD97 levels correlating with migration and invasion in vitro. CD97 is also expressed in various gastric, pancreatic and esophageal carcinomas. CD97 shares significant homology with EMR2, however the two proteins exhibit different expression patterns, as EMR2 is not expressed in any of the aforementioned cancer cells.
• Source: E. coli
• Species: Human
• Tag: C-His
• Molecular Mass: ~59 kDa
• AA Sequence: QDSRGCARWCPQNSSCVNATACRCN PGFSSFSEIITTPTETCDDINECAT PSKVSCGKFSDCWNTEGSYDCVCSP GYEPVSGAKTFKNESENTCQDVDEC QQNPRLCKSYGTCVNTLGSYTCQCL PGFKFIPEDPKVCTDVNECTSGQNP CHSSTHCLNNVGSYQCRCRPGWQPI PGSPNGPNNTVCEDVDECSSGQHQC DSSTVCFNTVGSYSCRCRPGWKPRH GIPNNQKDTVCEDMTFSTWTPPPGV HSQTLSRFFDKVQDLGRDSKTSSAE VTIQNVIKLVDELMEAPGDVEALAP PVRHLIATQLLSNLEDIMRILAKSL PKGPFTYISPSNTELTLMIQERGDK NVTMGQSSARMKLNWAVAAGAEDPG PAVAGILSIQNMTTLLANASLNLHS KKQAELEEIYESSIRGVQLRRLSAV NSIFLSHNNTKELNSPILFAFSHLE SSDGEAGRDPPAKDVMPGPRQELLC AFWKSDSDRGGHWATEGCQVLGSKN GSTTCQCSHLSSFAILMAHYDVEDW KLTLITR
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: ADGRE5 adhesion G protein-coupled receptor E5 [ Homo sapiens (human) ]
• Official Symbol: ADGRE5
• Synonyms: ADGRE5; adhesion G protein-coupled receptor E5; CD97; TM7LN1; adhesion G protein-coupled receptor E5; CD97 molecule; leukocyte antigen CD97; seven transmembrane helix receptor; seven-span transmembrane protein; seven-transmembrane, heterodimeric receptor associated with inflammation
• Gene ID: 976
• mRNA Refseq: NM_001784
• Protein Refseq: NP_001775
• MIM: 601211
• UniProt ID: P48960

Reviews

09/26/2021

Highly effective in detecting and measuring the activity of esterases in esterase assays.

07/27/2021

Shows excellent performance in detecting and measuring the activity of phosphatases in phosphatase assays.

Q&As

What are the downstream signaling pathways activated by ADGRE5 and how do they contribute to cellular processes?

ADGRE5 activates specific downstream signaling pathways, including [specific signaling cascades], which contribute to various cellular processes, such as [cell migration, invasion, or adhesion].

Can the expression or activity of ADGRE5 be modulated by pharmacological agents or genetic manipulation?

The expression or activity of ADGRE5 can be modulated by pharmacological agents that target [specific receptors or signaling pathways], or through genetic manipulation using [specific genetic tools or techniques].

Are there any known ligands or interacting partners that bind to the ADGRE5 protein and modulate its function?

ADGRE5 interacts with specific ligands or interacting partners, such as [specific molecules or cell surface receptors], leading to the modulation of its function.

Does ADGRE5 play a role in cell migration, invasion, or adhesion, and what are the underlying mechanisms?

ADGRE5 plays a role in cell migration, invasion, and adhesion through mechanisms involving [specific cellular processes or adhesion molecules].

What are the functional consequences of ADGRE5 activation or inhibition on cellular processes and disease progression?

Activation or inhibition of ADGRE5 has functional consequences on cellular processes and disease progression, impacting [specific cellular activities or disease outcomes].

Does ADGRE5 play a role in specific diseases or pathological conditions, and if so, what are the associated mechanisms?

ADGRE5 is implicated in specific diseases or pathological conditions, such as [specific cancer types or inflammatory disorders], where it contributes to [specific disease mechanisms or cellular dysregulation].

What is the tissue-specific expression pattern of the ADGRE5 protein across different organs and cell types?

The ADGRE5 protein exhibits a specific expression pattern across various tissues and cell types, with higher levels observed in [specific tissues or cell populations].

How is the expression of ADGRE5 regulated under normal physiological conditions and in response to specific stimuli?

Under normal physiological conditions, the expression of ADGRE5 is tightly regulated and can be modulated in response to specific stimuli, such as [specific cytokines or extracellular cues].

What is the impact of ADGRE5 deficiency or knockout on cell phenotypes or relevant biological processes?

Deficiency or knockout of ADGRE5 in cells leads to observable changes in cell phenotypes or altered biological processes, characterized by [specific cellular dysfunctions or functional impairments].

What is the subcellular localization and distribution pattern of ADGRE5 within cells or relevant cell types?

ADGRE5 exhibits a [specific subcellular localization pattern], with localization primarily observed in [specific cellular compartments, such as plasma membrane or endosomes].