Recombinant Human ADP-ribosylation Factor 6, His-tagged

• Cat.No.: ARF6-3369H
• Product Overview: Recombinant human ARF6 protein , fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 22.2 kDa (195aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKL KLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKIRPLWRHYYTG TQGLIF VV DC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGDGLYEGLTWLT SNYKS.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Fuction: GTP binding; GTPase activity; nucleotide binding; protein binding; thioesterase binding
• Pathways: Endocytosis; Fc gamma R-mediated phagocytosis

Gene Information

• Gene Name: ARF6 ADP-ribosylation factor 6 [ Homo sapiens ]
• Synonyms: ADP-ribosylation factor 6; DKFZp564M0264; ARF6
• Gene ID: 382
• mRNA Refseq: NM_001663
• Protein Refseq: NP_001654
• MIM: 600464
• UniProt ID: P62330
• Chromosome Location: 14q21.3