"ARF6" Related Products

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Recombinant Human ARF6 protein, His-tagged

Cat.No.: ARF6-26533TH
Product Overview: Recombinant Human ARF6(1-175 aa) fused with His tag was expressed in E. coli.
Description: ADP-ribosylation factor 6, also known as ARF6, is a member of the ADP ribosylation factor family of GTP-binding proteins. It is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. Additionally, ARF6 play a central role in maintenance of organelle integrity, assembly of coat proteins and activation of phospholipase D. Recombinant human ARF6 protein , fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 0.2mM PMSF
Molecular Mass: 22.2 kDa (195aa) confirmed by MALDI-TOF
AA Sequence: MGSSHHHHHH SSGLVPRGSHRPLWRHYYTG TQGLIFVVDC ADRDRIDEAR QELHRIINDR EMRDAIILIF ANKQDLPDAM KPHEIQEKLG LTRIRDRNWY VQPSCATSGDMGKVLSKIFG NKEMRILMLG LDAAGKTTIL YKLKLGQSVT TIPTVGFNVE TVTYKNVKFN VWDVGGQDKI GLYEGLTWLT SNYKS
Purity: > 95% by SDS - PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: ARF6 ADP-ribosylation factor 6 [ Homo sapiens ]
Official Symbol: ARF6
Synonyms: ARF6; ADP-ribosylation factor 6; DKFZp564M0264;
Gene ID: 382
mRNA Refseq: NM_001663
Protein Refseq: NP_001654
MIM: 600464
UniProt ID: P62330
Chromosome Location: 14q21.3
Pathway: Arf6 downstream pathway, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem; Arf6 trafficking events, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; E-cadherin signaling in the nascent adherens junction, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem;
Function: GTP binding; GTPase activity; nucleotide binding; protein binding; thioesterase binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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