Recombinant Human ADRBK2 Protein, GST-tagged

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Recombinant Human ADRBK2 Protein, GST-tagged

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Cat.No. : ADRBK2-385H
Product Overview : Human ADRBK2 (CAB45657.1, 1 a.a. - 688 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Description : The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]
Source : Insect Cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was determined by ELISA. The enzyme was incubated with biotinylated substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into streptavidin- coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG. Substrate: Porcine tublin. ATP: 100 uM.
Molecular Mass : 107 kDa
Purity : 51 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing: :

Loading 1 ug protein in SDS-PAGE

Result of activity analysis :

Gene Name : ADRBK2 adrenergic, beta, receptor kinase 2 [ Homo sapiens ]
Official Symbol : ADRBK2
Synonyms : ADRBK2; adrenergic, beta, receptor kinase 2; beta-adrenergic receptor kinase 2; BARK2; GRK3; beta-ARK-2; G-protein-coupled receptor kinase 3;
Gene ID : 157
mRNA Refseq : NM_005160
Protein Refseq : NP_005151
MIM : 109636
UniProt ID : P35626

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