Recombinant Human AGR3 Protein, Fc-tagged

• Cat.No.: AGR3-577H
• Product Overview: Recombinant human AGR3 (NP_789783.1) (Met1-Leu166) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Predicted N Terminal: Ile 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant human AGR3 consists 379 amino acids and predicts a molecular mass of 43.4 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: AGR3 anterior gradient 3 homolog (Xenopus laevis) [ Homo sapiens ]
• Official Symbol: AGR3
• Synonyms: AGR3; anterior gradient 3 homolog (Xenopus laevis); anterior gradient protein 3 homolog; BCMP11; breast cancer membrane protein 11; hAG 3; HAG3; PDIA18; protein disulfide isomerase family A; member 18; AG-3; anterior gradient homolog 3; protein disulfide isomerase family A, member 18; AG3; hAG-3
• Gene ID: 155465
• mRNA Refseq: NM_176813
• Protein Refseq: NP_789783
• MIM: 609482
• UniProt ID: Q8TD06
• Chromosome Location: 7p21.1

Reviews

06/20/2022

I would appreciate more detailed documentation accompanying the product.

04/23/2021

The proteins offered by this company are of exceptional quality and come at an affordable price, making them an excellent choice for my research needs.

07/22/2020

The customer service was exceptional, with prompt assistance and valuable technical support.

10/22/2019

This product exceeded my expectations in terms of quality.

05/04/2019

This protein helo me to continue my experiments without breaking the bank.

Q&As

How can the interaction partners of AGR3 be identified in the laboratory?

The interaction partners of AGR3 can be identified using techniques such as co-immunoprecipitation (Co-IP), yeast two-hybrid assays, or proximity-dependent biotin identification (BioID).

How does AGR3 contribute to cancer development?

AGR3 is thought to promote cancer cell survival, proliferation, and metastasis by modulating cellular signaling pathways and interacting with other proteins involved in cancer progression.

What are the molecular mechanisms underlying AGR3's role in cancer?

The exact mechanisms are still being elucidated, but AGR3 has been shown to interact with proteins involved in cell adhesion, invasion, and apoptosis, contributing to cancer phenotypes.

What are the recommended storage conditions for AGR3 protein samples in the laboratory?

AGR3 protein samples should be stored at -80°C to maintain their stability and prevent degradation. It is advisable to aliquot the protein to avoid repeated freeze-thaw cycles.

What are the common techniques used to purify AGR3 protein in the laboratory?

The purification of AGR3 protein can be achieved through techniques such as affinity chromatography, size-exclusion chromatography, or ion-exchange chromatography.

What is the relationship between AGR3 and androgen signaling in prostate cancer?

AGR3 expression is regulated by androgen receptor signaling in prostate cancer cells, and it may play a role in mediating androgen-dependent growth and survival.

Can AGR3 be targeted for therapeutic interventions?

Targeting AGR3 is an area of active research, and potential strategies such as small molecule inhibitors or immunotherapies are being explored for therapeutic interventions.

How can AGR3 expression be measured in experimental studies?

AGR3 expression can be detected and quantified using techniques such as immunohistochemistry, immunoblotting, and molecular assays like quantitative PCR (qPCR) or RNA sequencing.

Are there any animal models available to study AGR3 function?

Limited animal models, such as mouse models, have been utilized to study AGR3 function in specific tissues or diseases, providing insights into its physiological roles.

Does AGR3 have any extracellular functions?

Similar to AGR2, AGR3 can be secreted into the extracellular space, where it may interact with other proteins and potentially influence cell signaling and behavior.