Recombinant Human AIMP1 protein, His-tagged

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Recombinant Human AIMP1 protein, His-tagged

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Cat.No. : AIMP1-345H
Product Overview : Recombinant Human AIMP1 protein (Ala2-Lys312), fused to His tag at N-terminus, was expressed in E.coli.
Description : Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 (AIMP1) is also known as multisynthase complex auxiliary component p43 and endothelial monocyte-activating polypeptide II (EMAP-II). AIMP1 is a cytokine that may be induced by apoptosis and is also released from professional antigen-presenting cells such as dendritic cells. The release of AIMP1 renders the tumor-associated vasculature sensitive to tumor necrosis factor. Furthermore, AIMP1 binds tRNA and stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. This protein possesses inflammatory cytokine activity. Also, AIMP1 negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation.
Source : E.coli
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 35.2 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE).
Protein length : 311
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of AIMP1-345H :

Human AIMP1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : AIMP1
Official Symbol : AIMP1
Synonyms : AIMP1; aminoacyl tRNA synthetase complex-interacting multifunctional protein 1; SCYE1, small inducible cytokine subfamily E, member 1 (endothelial monocyte activating); aminoacyl tRNA synthase complex-interacting multifunctional protein 1; ARS interacting multifunctional protein 1; EMAP II; EMAP 2; EMAPII; p43; ARS-interacting multifunctional protein 1; endothelial monocyte-activating polypeptide 2; multisynthase complex auxiliary component p43; endothelial-monocyte activating polypeptide II; multisynthetase complex auxiliary component p43; small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating); HLD3; EMAP2; SCYE1
Gene ID : 9255
mRNA Refseq : NM_001142415
Protein Refseq : NP_001135887
MIM : 603605
UniProt ID : Q12904

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