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Recombinant Human AKT1 protein, Flag-tagged, Biotinylated

Cat.No.: AKT1-30H
Product Overview: Recombinant Human AKT1, catalytic domain [104-480(end) amino acids of accession number NP_005154.1] fused with DYKDDDDK tag at N-terminal was expressed in insect cell and biotinylated.
Source: Insect cell
Species: Human
Tag: Flag
Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
Molecular Mass: 47 kDa
Purity: >87% as determined by SDS-PAGE and CBB staining
Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Gene Name: AKT1 v-akt murine thymoma viral oncogene homolog 1 [ Homo sapiens ]
Official Symbol: AKT1
Synonyms: AKT1; AKT; PKB; PRKBA; RAC; PKB alpha; RAC-PK-alpha
Gene ID: 207
mRNA Refseq: NM_001014431
Protein Refseq: NP_001014431
MIM: 164730
UniProt ID: P31749
Chromosome Location: 14q32.32-q32.33
Pathway: AKT phosphorylates targets in the cytosol, organism-specific biosystem; AKT phosphorylates targets in the nucleus, organism-specific biosystem; AKT-mediated inactivation of FOXO1A, organism-specific biosystem; Activation of BAD and translocation to mitochondria, organism-specific biosystem; Activation of BH3-only proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem;
Function: ATP binding; ATP binding; enzyme binding; identical protein binding; kinase activity; nitric-oxide synthase regulator activity; nucleotide binding; phosphatidylinositol-3,4,5-trisphosphate binding; phosphatidylinositol-3,4-bisphosphate binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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