Recombinant Human ALAD Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human ALAD Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : ALAD-6108H
Product Overview : ALAD MS Standard C13 and N15-labeled recombinant protein (NP_000022) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 37.23 kDa
AA Sequence : MPLCPLAHAM QPQSVLHSGY FHPLLRAWQT ATTTLNASNL IYPIFVTDVP DDIQPITSLP GVARYGVKRL EEMLRPLVEE GLRCVLIFGV PSRVPKDERG SAADSEESPA IEAIHLLRKT FPNLLVACDV CLCPYTSHGH CGLLSENGAF RAEESRQRLA EVALAYAKAG CQVVAPSDMM DGRVEAIKEA LMAHGLGNRV SVMSYSAKFA SCFYGPFRDA AKSSPAFGDR RCYQLPPGAR GLALRAVDRD VREGADMLMV KPGMPYLDIV REVKDKHPDL PLAVYHVSGE FAMLWHGAQA GAFDLKAAVL EAMTAFRRAG ADIIITYYTP QLLQWLKEES GPTRTRRLEQ KLISEEDLAA NDILDYKDDD DKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : ALAD aminolevulinate dehydratase [ Homo sapiens (human) ]
Official Symbol : ALAD
Synonyms : ALAD; aminolevulinate dehydratase; aminolevulinate, delta, dehydratase; delta-aminolevulinic acid dehydratase; ALADH; PBGS; porphobilinogen synthase; aminolevulinate, delta-, dehydratase; MGC5057;
Gene ID : 210
mRNA Refseq : NM_000031
Protein Refseq : NP_000022
MIM : 125270
UniProt ID : P13716

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